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Beclin-1 表达是淋巴结阳性胃癌患者生存的重要预测指标。

Beclin-1 expression is a significant predictor of survival in patients with lymph node-positive gastric cancer.

机构信息

State Key Laboratory of Oncology in South China, Guangzhou, China.

出版信息

PLoS One. 2012;7(9):e45968. doi: 10.1371/journal.pone.0045968. Epub 2012 Sep 27.

DOI:10.1371/journal.pone.0045968
PMID:23029344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3459952/
Abstract

BACKGROUND

Beclin 1 is a main actor of autophagy. The expression of Beclin 1 and its prognostic role in gastric cancer is largely unexplored. The purpose of the present study is to investigate the expression of beclin 1 in gastric cancer cells, tissues and its relationship with prognosis.

METHODS

The expression of Beclin 1 was detected in 271 specimens of lymph-node positive gastric cancer patients by immunohistochemistry. The correlation of Beclin 1 expression to clinicopathologic features and survival of gastric cancer was studied. Beclin-1 expression in gastric cancer cell lines and clinical specimens is also detected using reverse transcription-PCR and Western blotting.

RESULTS

Beclin 1 is up-regulated at both mRNA and protein levels in six gastric cancer cell lines compared with those in normal gastric mucosa cell line (GES-1). The expression of Beclin-1 in gastric clinical specimens is also higher than those in the adjacent noncancerous tissues. Of the 271 patients, 229 (84.5%) were Beclin 1 high expression tumors by immunohistochemistry. Beclin 1 expression is closely associated with intravascular embolus. Kaplan-Meier analysis showed high beclin 1 expression was associated with longer overall survival. Both univariate analysis and multivariate analysis revealed that Beclin 1 expression were independent prognostic factors in the patients with node-positive gastric cancer.

CONCLUSIONS

Our findings strongly suggest that Beclin 1 has a potential role in tumorigenesis of gastric cancer and could be a promising biomarker for predicting the prognosis of patients with lymph node-positive gastric cancer. It might also serve as a novel therapeutic target for gastric cancer treatment.

摘要

背景

Beclin 1 是自噬的主要作用因子。Beclin 1 在胃癌中的表达及其预后作用在很大程度上仍未被探索。本研究旨在探讨 Beclin 1 在胃癌细胞、组织中的表达及其与预后的关系。

方法

采用免疫组织化学方法检测 271 例淋巴结阳性胃癌患者标本中 Beclin 1 的表达，研究 Beclin 1 表达与胃癌临床病理特征及生存的关系。采用逆转录-PCR 和 Western blot 检测胃癌细胞系和临床标本中 Beclin-1 的表达。

结果

与正常胃黏膜细胞系（GES-1）相比，在 6 株胃癌细胞系中 Beclin 1 在 mRNA 和蛋白水平上均上调。胃癌临床标本中 Beclin-1 的表达也高于相邻的非癌组织。在 271 例患者中，229 例（84.5%）免疫组织化学检测为 Beclin 1 高表达肿瘤。Beclin 1 的表达与血管内栓子密切相关。Kaplan-Meier 分析显示，高 Beclin 1 表达与总生存期延长相关。单因素和多因素分析均表明，Beclin 1 表达是淋巴结阳性胃癌患者的独立预后因素。

结论

我们的研究结果强烈表明，Beclin 1 在胃癌的肿瘤发生中具有潜在作用，可能成为预测淋巴结阳性胃癌患者预后的有前途的生物标志物。它也可能成为胃癌治疗的新的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/47d8920d1335/pone.0045968.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/73694d936b18/pone.0045968.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/cb5b699a86fb/pone.0045968.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/b3176b19bf74/pone.0045968.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/47d8920d1335/pone.0045968.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/73694d936b18/pone.0045968.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/cb5b699a86fb/pone.0045968.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/b3176b19bf74/pone.0045968.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a0/3459952/47d8920d1335/pone.0045968.g004.jpg

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