小鼠α1-抗胰蛋白酶的完整cDNA序列及染色体定位

Complete cDNA sequence and chromosomal localization of mouse alpha 1-antitrypsin.

作者信息

Sifers R N, Ledley F D, Reed-Fourquet L, Ledbetter D H, Ledbetter S A, Woo S L

机构信息

Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.

出版信息

Genomics. 1990 Jan;6(1):100-4. doi: 10.1016/0888-7543(90)90453-2.

DOI:10.1016/0888-7543(90)90453-2

PMID:2303252

Abstract

A cDNA encoding the complete open reading frame of murine alpha 1-antitrypsin has been cloned and sequenced. The nucleic acid and predicted amino acid sequences show homology to human alpha 1-antitrypsin and demonstrate the preservation of critical structural determinants for intracellular targeting, carbohydrate attachment, and catalytic function. The alpha 1-antitrypsin gene locus (Aat) has been localized on murine chromosome 12E----F by in situ hybridization.

摘要

编码小鼠α1-抗胰蛋白酶完整开放阅读框的cDNA已被克隆和测序。核酸序列和预测的氨基酸序列显示与人类α1-抗胰蛋白酶具有同源性，并证明了对于细胞内靶向、碳水化合物附着和催化功能的关键结构决定簇得以保留。通过原位杂交，α1-抗胰蛋白酶基因位点（Aat）已定位在小鼠第12号染色体的E----F区域。