小鼠GABAAα1受体基因的cDNA序列及染色体定位

The cDNA sequence and chromosomal location of the murine GABAA alpha 1 receptor gene.

作者信息

Keir W J, Kozak C A, Chakraborti A, Deitrich R A, Sikela J M

机构信息

Department of Pharmacology, Alcohol Research Center, University of Colorado Health Sciences Center, Denver.

出版信息

Genomics. 1991 Feb;9(2):390-5. doi: 10.1016/0888-7543(91)90272-g.

DOI:10.1016/0888-7543(91)90272-g

PMID:1848528

Abstract

The murine GABAA/benzodiazepine (GABAA/BZ) receptor alpha 1 subunit cDNA has been isolated from a BALB/c mouse brain library and sequenced. The cDNA is 2665 nucleotides long with an open reading frame of 455 amino acids. It shows significant homology to the GABAA receptor alpha 1 subunit cDNA sequences of other species. Excluding deletions, the murine GABAA alpha 1 receptor exhibits 96% nucleotide and 100% amino acid sequence homology to the rat alpha 1 receptor cDNA and over 91% nucleotide and 98% amino acid sequence homology to the bovine and human alpha 1 receptor cDNAs in the protein coding region. This murine cDNA was used to locate the alpha 1 receptor subunit gene, Gabra-1, to murine Chromosome 11 between Il-3 and Rel. This assignment extends proximally the segment of mouse Chromosome 11 with known homology to human chromosome 5.

摘要

已从小鼠脑文库中分离出小鼠γ-氨基丁酸A型/苯二氮䓬（GABAA/BZ）受体α1亚基的互补DNA（cDNA）并进行了测序。该cDNA长2665个核苷酸，具有一个455个氨基酸的开放阅读框。它与其他物种的GABAA受体α1亚基cDNA序列具有显著同源性。排除缺失情况，小鼠GABAAα1受体在核苷酸水平上与大鼠α1受体cDNA的同源性为96%，在蛋白质编码区与牛和人类α1受体cDNA的核苷酸同源性超过91%，氨基酸序列同源性为98%。该小鼠cDNA被用于将α1受体亚基基因Gabra-1定位到小鼠第11号染色体上Il-3和Rel之间的区域。这一定位将小鼠第11号染色体上与人类5号染色体具有已知同源性的片段向近端延伸。