Magnani Karla Luciana, Cataneo Daniele Cristina, Capelozzi Vera Luiza, Defaveri Julio, Hasimoto Erica Nishida, Cataneo Antônio José Maria
Botucatu School of Medicine, UNESP, Sao Paulo, Brazil.
Acta Cir Bras. 2012 Oct;27(10):687-93. doi: 10.1590/s0102-86502012001000004.
Investigate the morphological effects of chronic exposure to tobacco smoke inhalation and alcohol consumption on the lungs and on the growth of rats.
Sixty male Wistar rats were divided into four groups: control, tobacco, alcohol, tobacco + alcohol, for a period of study 260 days. Morphological analysis was conducted by optical and electron microscopy. Rat growth was investigated by measuring the snout-anus length, body mass index and body weight.
The three groups exposed to the drugs presented lower growth and lower weight than the control group. The percentages of alveolitis, bronchiolitis and the mean alveolar diameter were greater, particularly in the groups exposed to tobacco smoke, but were not significantly different from the control group. Electron microscopy revealed more intense apoptotic and degenerative lesions in the smoking group, while degenerative lesions in the lamellar bodies were more intense with the association of both drugs.
This experimental model showed morphological alterations observed by electron microscopy, principally due to tobacco smoke exposure. Alcohol and tobacco hindered the growth of rats, such that tobacco showed a greater effect on body length and alcohol on body weight.
研究长期吸入烟草烟雾和饮酒对大鼠肺部及生长的形态学影响。
将60只雄性Wistar大鼠分为四组：对照组、烟草组、酒精组、烟草+酒精组，研究期为260天。通过光学显微镜和电子显微镜进行形态学分析。通过测量口鼻-肛门长度、体重指数和体重来研究大鼠生长情况。
与对照组相比，接触药物的三组大鼠生长较慢且体重较低。肺泡炎、细支气管炎的百分比以及平均肺泡直径更大，尤其是在接触烟草烟雾的组中，但与对照组无显著差异。电子显微镜显示吸烟组凋亡和退行性病变更明显，而两种药物联合使用时板层小体的退行性病变更明显。
该实验模型显示通过电子显微镜观察到形态学改变，主要是由于接触烟草烟雾所致。酒精和烟草阻碍了大鼠的生长，其中烟草对体长影响更大，酒精对体重影响更大。
