Antibody production and tissue irritation in swine vaccinated with Actinobacillus bacterins containing various adjuvants.

The effects that Actinobacillus pleuropneumoniae bacterins containing paraffin (mineral) oil, Al(OH)3, or lecithin-base oil adjuvants had on antibody production and muscle irritation were studied. Four hundred and thirty-two pigs were vaccinated twice (864 injection sites) at a 30-day interval. To assess effects of skin surface contamination on tissue reaction, 247 of the injections were made through skin soiled with manure. Serum samples for antibody determination and tissue samples were obtained at slaughter (15 to 90 days after vaccination). Bacterins containing Al(OH)3 or 5% lecithin-base oil as an adjuvant resulted in minimal tissue irritation. Bacterins containing paraffin oil or greater than 20% lecithin-base oil were more irritating to muscle, often resulting in a granuloma or an abscess at the injection site. Pigs given bacterins containing Al(OH)3 adjuvant had lower antibody titers than did pigs given bacterins containing paraffin oil or lecithin-base oil adjuvants.