College of Life Sciences, Wuhan University, Wuhan, Hubei Province, P R China.
Mol Nutr Food Res. 2012 Nov;56(11):1729-38. doi: 10.1002/mnfr.201200316. Epub 2012 Oct 4.
Mushrooms are well known for their nutritional and medicinal value. Agrocybe aegerita has been used as a nutritious food around the world and for its herbal medicinal properties in Asia. In recent years, several antitumor proteins have been identified from A. aegerita. The objective of this study was to purify a novel antitumor protein from A. aegerita.
A novel antitumor protein A. aegerita deoxyribonuclease (AAD) was purified through a two-step chromatographic procedure and was shown to possess antitumor activity against different cancer cell lines. Cells treated with AAD produced typical apoptotic morphological changes, which include chromatin condensation, the accumulation of sub-G1 cells and caspase-8 cleavage. Biochemical characterization of AAD showed that it was a member of the DNase I family and that it possessed divalent metal ion-dependent endonuclease activity. The optimal temperature for AAD activity was 50°C and its optimal pH was 8.5. The MS-identified peptides of AAD were found to match to Unigene3821, which has 97% homology with Aa-Pri1, in our A. aegerita transcriptome.
We have identified a novel antitumor protein from A. aegerita. This fuller understanding of A. aegerita would help us to enhance its use in nutritional and medical applications.
蘑菇以其营养价值和药用价值而闻名。金针菇在世界各地被用作营养食品，并因其在亚洲的草药药用特性而被使用。近年来，从金针菇中已经鉴定出几种抗肿瘤蛋白。本研究的目的是从金针菇中纯化一种新型抗肿瘤蛋白。
通过两步层析程序纯化了一种新型抗肿瘤蛋白金针菇脱氧核糖核酸酶（AAD），并证明其对不同癌细胞系具有抗肿瘤活性。用 AAD 处理的细胞产生典型的凋亡形态变化，包括染色质浓缩、亚 G1 细胞的积累和 caspase-8 的切割。AAD 的生化特性表明它是 DNA 酶 I 家族的成员，具有二价金属离子依赖性内切酶活性。AAD 的最佳温度为 50°C，最佳 pH 值为 8.5。AAD 的 MS 鉴定肽与我们金针菇转录组中的 Unigene3821 相匹配，其与 Aa-Pri1 的同源性为 97%。
我们已经从金针菇中鉴定出一种新型抗肿瘤蛋白。对金针菇的更深入了解将有助于我们增强其在营养和医疗应用中的应用。
