Mutyal Nikhil N, Radosevich Andrew, Gould Bradley, Rogers Jeremy D, Gomes Andrew, Turzhitsky Vladimir, Backman Vadim
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
Opt Express. 2012 Aug 27;20(18):19643-57. doi: 10.1364/OE.20.019643.
Low-coherence enhanced backscattering (LEBS) spectroscopy is an angular resolved backscattering technique that is sensitive to sub-diffusion light transport length scales in which information about scattering phase function is preserved. Our group has shown the ability to measure the spatial backscattering impulse response function along with depth-selective optical properties in tissue ex-vivo using LEBS. Here we report the design and implementation of a lens-free fiber optic LEBS probe capable of providing depth-limited measurements of the reduced scattering coefficient in-vivo. Experimental measurements combined with Monte Carlo simulation of scattering phantoms consisting of polystyrene microspheres in water are used to validate the performance of the probe. Additionally, depth-limited capabilities are demonstrated using Monte Carlo modeling and experimental measurements from a two-layered phantom.
低相干增强背散射（LEBS）光谱学是一种角分辨背散射技术，它对亚扩散光传输长度尺度敏感，在该尺度下关于散射相函数的信息得以保留。我们的团队已经展示了使用LEBS在体外测量组织中空间背散射脉冲响应函数以及深度选择性光学特性的能力。在此，我们报告一种无透镜光纤LEBS探头的设计与实现，该探头能够在体内提供对约化散射系数的深度受限测量。将实验测量结果与由水中聚苯乙烯微球组成的散射体模的蒙特卡罗模拟相结合，用于验证探头的性能。此外，使用蒙特卡罗建模以及来自双层体模的实验测量结果展示了深度受限能力。
