Wilke R N, Priebe M, Bartels M, Giewekemeyer K, Diaz A, Karvinen P, Salditt T
Institut für Röntgenphysik, Georg-August-Universität, Göttingen, Germany.
Opt Express. 2012 Aug 13;20(17):19232-54. doi: 10.1364/OE.20.019232.
Ptychographic coherent X-ray diffractive imaging (PCDI) has been combined with nano-focus X-ray diffraction to study the structure and density distribution of unstained and unsliced bacterial cells, using a hard X-ray beam of 6.2keV photon energy, focused to about 90nm by a Fresnel zone plate lens. While PCDI provides images of the bacteria with quantitative contrast in real space with a resolution well below the beam size at the sample, spatially resolved small angle X-ray scattering using the same Fresnel zone plate (cellular nano-diffraction) provides structural information at highest resolution in reciprocal space up to 2nm(-1). We show how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup. In addition, we present 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography.
叠层相干X射线衍射成像（PCDI）已与纳米聚焦X射线衍射相结合，用于研究未染色、未切片细菌细胞的结构和密度分布，使用的是光子能量为6.2keV的硬X射线束，通过菲涅耳波带片透镜聚焦至约90nm。虽然PCDI能在实空间中以定量对比度提供细菌图像，分辨率远低于样品处的束斑尺寸，但使用同一菲涅耳波带片进行的空间分辨小角X射线散射（细胞纳米衍射）能在倒易空间中提供高达2nm⁻¹的最高分辨率的结构信息。我们展示了如何在同一样品和同一装置上协同使用实空间和倒易空间方法。此外，我们还通过叠层成像和断层扫描相结合的方式展示了未染色细菌细胞的三维硬X射线成像。
