Riedel H H, Wellnitz K, Lehmann-Willenbrock E
Department of Gynecology, Rheinisch-Westfälische Technische-Hochschule Aachen, Federal Republic of Germany.
J Reprod Med. 1990 Feb;35(2):128-32.
The penetration rate of human spermatozoa in zona pellucida-free hamster oocytes was reduced by four monoclonal antisperm antibodies (A-24, B-20, III3 and VII-5) of six tested. The other two (VI-1 and VI-16) left the penetration rates virtually unchanged. The extent of the penetration rate reduction depended on the concentrations of antibodies A-24, B-20, III-3 and VII-5; if undiluted, they produced a significant reduction in the penetration rates; the largest average reduction was almost 50% with undiluted antibody A-24. Decreasing concentrations of the monoclonal antisperm antibodies resulted in significantly smaller reductions in the sperm penetration rates. The reason for the reduced penetration rates observed might have been a blockage of receptors on the sperm surface by the antibodies. That can inhibit enzyme reactions of the spermatozoa or interfere with the interaction between oocyte and spermatozoon membranes. Only antibody III-3 caused agglutination of human spermatozoa in the micro sperm agglutination and immobilization tests. Therefore, the agglutination or immobilization of spermatozoa might not be responsible for the reduced penetration rates after the addition of monoclonal antibodies, in contrast to findings with polyclonal antibodies.
在六种测试的单克隆抗精子抗体（A-24、B-20、III3和VII-5）中，有四种会降低人精子在无透明带仓鼠卵母细胞中的穿透率。另外两种（VI-1和VI-16）对穿透率几乎没有影响。穿透率降低的程度取决于抗体A-24、B-20、III-3和VII-5的浓度；如果不稀释，它们会显著降低穿透率；未稀释的抗体A-24导致的平均穿透率降低最大，几乎达到50%。单克隆抗精子抗体浓度降低，精子穿透率的降低也显著减小。观察到的穿透率降低的原因可能是抗体阻断了精子表面的受体。这会抑制精子的酶反应或干扰卵母细胞与精子细胞膜之间的相互作用。在微精子凝集和制动试验中，只有抗体III-3会导致人精子凝集。因此，与多克隆抗体的研究结果相反，添加单克隆抗体后精子的凝集或制动可能不是穿透率降低的原因。
