Institute of Medical Biology, 8A Biomedical Grove, Immunos 138648, Singapore.
Cell Stem Cell. 2012 Oct 5;11(4):452-60. doi: 10.1016/j.stem.2012.09.009.
Decades ago, two nonoverlapping crypt stem cell populations were proposed: Leblond's Crypt Base Columnar (CBC) cell and Potten's +4 cell. The identification of CBC markers including Lgr5 has confirmed Leblond's predictions that CBC cells are anatomically distinct, long-lived stem cells that permanently cycle. While Potten originally described +4 cells as proliferative and unusually radiation-sensitive, recent efforts to identify +4 stem cells have focused on the identification of cells that are quiescent and radiation-resistant. Here, we describe commonalities and discrepancies between the individual studies and discuss challenges of marker-based lineage tracing.
几十年前，人们提出了两个不重叠的隐窝干细胞群体：Leblond 的隐窝基柱状（CBC）细胞和 Potten 的+4 细胞。包括 Lgr5 在内的 CBC 标志物的鉴定证实了 Leblond 的预测，即 CBC 细胞在解剖上是不同的，是具有永久自我更新能力的长寿干细胞。虽然 Potten 最初将+4 细胞描述为增殖和异常辐射敏感，但最近鉴定+4 干细胞的努力集中在鉴定静止和辐射抗性细胞上。在这里，我们描述了各个研究之间的相似性和差异，并讨论了基于标记的谱系追踪的挑战。
