Feng Yuqing, Zeng Nan, Bordbar Farhad, Lu Zhujin, Gao Chunqi
College of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Laboratory for Lingnan Modern Agriculture, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural University, Guangzhou 510642, China.
College of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Laboratory for Lingnan Modern Agriculture, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural University, Guangzhou 510642, China.
Poult Sci. 2025 Feb;104(2):104821. doi: 10.1016/j.psj.2025.104821. Epub 2025 Jan 14.
This study aimed to investigate the effects of dietary supplements of fermented mixed ingredient product (FMIP) on the growth performance, intestinal health, and immune performance of layer hens during the brooding period. Four hundred eighty healthy one-day-old layer chicks were randomly divided into four groups (six replicates/group, twenty hens/replicate) and were fed with different experimental diets for eight weeks (from day 1 to day 56): (1) Corn-soybean-base diet (CON); (2) Chlortetracycline group (CTC; CON diet supplemented with 0.5g/kg chlortetracycline); (3) 4 % fermented mixed ingredient product (4 % FMIP); (4) 8 % fermented mixed ingredient product (8 % FMIP). The results showed that, compared with the CON group, feeding with CTC, 4 % or 8 % FMIP increased the average daily feed intake (ADFI), average daily gain (ADG), immune organs index, serum IgA, IgM, and IgG levels, as well as the apparent metabolic rates of dry matter, crude protein, crude fiber, and crude ash (P < 0.05). Meanwhile, FMIP supplementation improved jejunal morphology and barrier function, as reflected by increased villus height and transepithelial electrical resistance, decreased DAO activity in serum, and up-regulated Occludin protein expression (P < 0.05). Additionally, FMIP supplementation significantly increased protein expression of the stem cell markers (SOX9 and Lgr5), proliferative cell marker (PCNA), and differentiated absorptive cell marker (Villin) (P < 0.05). The immunofluorescence results were consistent with the above results, and FMIP groups have the same effects as the CTC group. Furthermore, the CTC or 4 % FMIP treatment group resulted in a remarkable increase in Wnt/β-catenin signaling proteins (including β-catenin, TCF4, c-Myc, and Cyclin-D1) compared with the CON group (P < 0.05). In conclusion, dietary supplementation of 4 % FMIP improves growth and immune performance, and promotes the intestinal stem cell expansion of layer chicks through Wnt/β-catenin pathway activation.
本研究旨在探讨日粮添加发酵混合成分产品（FMIP）对育雏期蛋鸡生长性能、肠道健康和免疫性能的影响。480只健康的1日龄蛋鸡雏随机分为四组（每组6个重复，每个重复20只母鸡），并饲喂不同的试验日粮8周（从第1天到第56天）：（1）玉米-大豆基础日粮（CON）；（2）金霉素组（CTC；CON日粮添加0.5g/kg金霉素）；（3）4%发酵混合成分产品（4%FMIP）；（4）8%发酵混合成分产品（8%FMIP）。结果表明，与CON组相比，饲喂CTC、4%或8%FMIP可提高平均日采食量（ADFI）、平均日增重（ADG）、免疫器官指数、血清IgA、IgM和IgG水平，以及干物质、粗蛋白、粗纤维和粗灰分的表观代谢率（P<0.05）。同时，添加FMIP改善了空肠形态和屏障功能，表现为绒毛高度增加和跨上皮电阻增加、血清中DAO活性降低以及闭合蛋白表达上调（P<0.05）。此外，添加FMIP显著增加了干细胞标志物（SOX9和Lgr5）、增殖细胞标志物（PCNA）和分化吸收细胞标志物（Villin）的蛋白表达（P<0.05）。免疫荧光结果与上述结果一致，FMIP组与CTC组具有相同的效果。此外，与CON组相比，CTC或4%FMIP处理组导致Wnt/β-连环蛋白信号蛋白（包括β-连环蛋白、TCF4、c-Myc和细胞周期蛋白D1）显著增加（P<0.05）。总之，日粮添加4%FMIP可改善蛋鸡雏的生长和免疫性能，并通过激活Wnt/β-连环蛋白途径促进肠道干细胞扩增。
