Liu Shun, Qiu Xiao-qiang, Zeng Xiao-yun, Bai Hua, Bei Chun-hua, Yang Yan
Department of Epidemiology and Statistics, Guangxi Medical University, Nanning, China.
Zhonghua Gan Zang Bing Za Zhi. 2012 Jun;20(6):463-7. doi: 10.3760/cma.j.issn.1007-3418.2012.06.017.
To study the relationship between the interleukin (IL)6 -572G/C polymorphism and risk of hepatocellular carcinoma (HCC) in men.A hospital-based case-control study was conducted with 500 male HCC patients without tumor history in other organs and 590 healthy male controls without history of tumors or chronic diseases. All HCC cases were diagnosed by histopathology. The controls were recruited from the Department of Orthopedic Trauma and Ophthalmology at the same hospital. The IL-6 promoter -572G/C polymorphism and its genotype variants were detected by real-time fluorescence quantitative PCR. The Chi-squared test and unconditional logistic regression analyses were applied to determine the risk of HCC among men carrying the different genotype variants.The frequencies of alleles and distribution of genotypes in the -572G/C loci were not significantly different between the HCC cases and controls (P more than 0.05). The Chi-squared test indicated that the polymorphisms of the loci were not associated with HCC in our male population. However, after adjusting by multivariate logistic regression, the odds ratio (OR) of HCC for the G allele (CG + GG genotypes) carriers was 1.31 (95% confidence interval (CI): 1.00 - 1.71) compared with the CC genotype. Among the male HBV carriers, the CG genotype increased HCC risk significantly (OR = 1.60, 95% CI: 1.14 - 2.24) compared with the CC genotype. A trend test indicated that HCC risk was significantly increased with the numbers of G alleles (P trend less than 0.05). Breslow-Day tests of homogeneity of the ORs indicated an interaction between hepatitis B virus (HBV) infection and polymorphisms of IL-6 (P less than 0.05). The synthetic odds ratio (OReg) of HBV infection and harboring a G allele was 5.95 (95% CI: 3.99-8.87), which represented a super multiplication interaction.Polymorphism of the IL-6 promoter -572 loci may be associated with HCC occurrence in men. Moreover, there is a super multiplication interaction for HCC risk between HBV infection and harboring the IL-6 G allele.
研究男性白细胞介素（IL）6 -572G/C基因多态性与肝细胞癌（HCC）风险之间的关系。开展了一项基于医院的病例对照研究，纳入500例无其他器官肿瘤病史的男性HCC患者以及590例无肿瘤或慢性病病史的健康男性对照。所有HCC病例均经组织病理学确诊。对照来自同一家医院的创伤骨科和眼科。采用实时荧光定量PCR检测IL-6启动子-572G/C基因多态性及其基因型变异。应用卡方检验和非条件logistic回归分析确定携带不同基因型变异的男性发生HCC的风险。HCC病例组与对照组之间-572G/C位点的等位基因频率和基因型分布无显著差异（P>0.05）。卡方检验表明，该位点的多态性与我们男性人群中的HCC无关。然而，经多因素logistic回归调整后，与CC基因型相比，G等位基因（CG + GG基因型）携带者发生HCC的比值比（OR）为1.31（95%置信区间（CI）：1.00 - 1.71）。在男性HBV携带者中，与CC基因型相比，CG基因型显著增加了HCC风险（OR = 1.60，95%CI：1.14 - 2.24）。趋势检验表明，HCC风险随G等位基因数量的增加而显著增加（P趋势<0.05）。OR的Breslow-Day齐性检验表明，乙型肝炎病毒（HBV）感染与IL-6多态性之间存在交互作用（P<0.05）。HBV感染和携带G等位基因的综合比值比（OReg）为5.95（95%CI：3.99 - 8.87），代表超相乘交互作用。IL-6启动子-572位点的多态性可能与男性HCC的发生有关。此外，HBV感染与携带IL-6 G等位基因之间在HCC风险方面存在超相乘交互作用。
