Hong Paul, Bance Manohar, Gratzer Paul F
IWK Health Centre, Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, Dalhousie University, Halifax, Nova Scotia, Canada.
Int J Pediatr Otorhinolaryngol. 2013 Jan;77(1):3-12. doi: 10.1016/j.ijporl.2012.09.022. Epub 2012 Oct 6.
To perform a contemporary review of experimental studies to describe the effects of various novel adjuvant therapies in enhancing tympanic membrane (TM) perforation healing.
A PubMed search for articles from January 2000 to June 2012 related to TM perforation, along with the references of those articles, was performed. Inclusion and exclusion criteria were applied to all experimental studies assessing adjuvant therapies to TM healing.
Many studies have assessed the efficacy of biomolecules or growth factors, such as epidermal growth factors and basic fibroblast growth factors, in TM regeneration with significant success. More recent strategies in TM tissue engineering have involved utilizing bioengineered scaffold materials, such as silk fibroin, chitosan, calcium alginate, and decellularized extracellular matrices. Most scaffold materials demonstrated biocompatibility and faster TM perforation healing rates.
Although several studies have demonstrated promising results, many questions still remain, such as the adequacy of animal models and long-term biocompatibility of adjuvant materials. As well, further studies comparing various adjuvant substances and bioscaffolds are required prior to clinical application.
对实验研究进行当代综述，以描述各种新型辅助疗法在促进鼓膜（TM）穿孔愈合方面的效果。
对2000年1月至2012年6月期间PubMed上与TM穿孔相关的文章及其参考文献进行检索。将纳入和排除标准应用于所有评估TM愈合辅助疗法的实验研究。
许多研究评估了生物分子或生长因子，如表皮生长因子和碱性成纤维细胞生长因子，在TM再生中的疗效，并取得了显著成功。TM组织工程中最近的策略涉及使用生物工程支架材料，如丝素蛋白、壳聚糖、海藻酸钙和脱细胞细胞外基质。大多数支架材料表现出生物相容性和更快的TM穿孔愈合率。
尽管多项研究已显示出有前景的结果，但仍存在许多问题，如动物模型的充分性和辅助材料的长期生物相容性。此外，在临床应用之前，需要进一步比较各种辅助物质和生物支架的研究。
