Favre H, Louis F, Gourjon M
Pflugers Arch. 1979 Oct;382(1):73-9. doi: 10.1007/BF00585907.
A previously described natriuretic factor found in urine from man and dogs receiving a high salt diet has been postulated to be of hormonal nature. In order to test this hypothesis, natriuretic factor obtained from a pool of human urine of a standard activity has been injected into rats fed 0.55 (group A), 3.55 (group B), 6.55 (group C) mEq sodium a day as it has been shown that the endogenous activity of this factor depends on the sodium content of the diet. This material induces an increment of the UNaV and FENa in the rats averaging respectively 429.6 +/- 150.1% and 317.5 +/- 71.14% for the rats from group A, 103 +/- 29% and 99.7 +/- 26.25% for the rats from group B, 56.3 +/- 34.4% and 63.2 +/- 35.8% for the rats from group C reflecting the degree of statistical significance observed between the absolute values obtained during the control and experimental periods for each group. In addition, a dose-response curve has been established. Altogether the results suggest the hormonal nature of the natriuretic factor and design the kidney as the target organ. These data allow to define the best conditions under which the rats have to be in order to provide a valuable bioassay for this natriuretic material.
在摄入高盐饮食的人和狗的尿液中发现的一种先前描述的利钠因子被推测具有激素性质。为了验证这一假设，已将从具有标准活性的人尿液池中获得的利钠因子注射到每天分别喂食0.55（A组）、3.55（B组）、6.55（C组）毫当量钠的大鼠体内，因为已表明该因子的内源性活性取决于饮食中的钠含量。这种物质使大鼠的尿钠排泄量（UNaV）和滤过钠排泄分数（FENa）增加，A组大鼠的平均增加量分别为429.6±150.1%和317.5±71.14%，B组大鼠为103±29%和99.7±26.25%，C组大鼠为56.3±34.4%和63.2±35.8%，这反映了每组在对照期和实验期获得的绝对值之间观察到的统计学显著程度。此外，还建立了剂量反应曲线。总体而言，结果表明利钠因子具有激素性质，并将肾脏确定为靶器官。这些数据有助于确定大鼠必须处于的最佳条件，以便为这种利钠物质提供有价值的生物测定。
