Brady L, Brzozowski A M, Derewenda Z S, Dodson E, Dodson G, Tolley S, Turkenburg J P, Christiansen L, Huge-Jensen B, Norskov L
Department of Chemistry, University of York, Heslington, UK.
Nature. 1990 Feb 22;343(6260):767-70. doi: 10.1038/343767a0.
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
真正的脂肪酶能附着三酰甘油并在油水界面起作用；它们是一类普遍存在的酶，催化各种各样的反应，其中许多具有工业应用潜力。但迄今为止，尚未有任何脂肪酶的三维结构被报道。在此，我们报告米黑根毛霉甘油三酯脂肪酶的X射线结构，并描述在3.1埃分辨率下获得并精修至1.9埃分辨率的原子模型。它揭示了一个丝氨酸..组氨酸..天冬氨酸类胰蛋白酶催化三联体，其中活性丝氨酸埋藏在一个长表面环的短螺旋片段之下。
