Bilgen Koksal, Bilgen Esra, Cetinkunar Suleyman, Celep R Bahadir, Isik Sevil
Ann Ital Chir. 2017;88:87-93.
We investigated the effıcacy of topical phenytoin and capsaicin on random pattern dorsal skin flaps in rats.
Twenty one Wistar rats were used in the study. Random-pattern McFarlane dorsal flaps 3 cm x 10 cm were raised in all the rats .A plastic barrier was placed between the flap and its donor site The flaps were sutured back to the original position with 4/0 nylon sutures. The rats were randomly divided into three groups (n=7). Group I was given only gel, Group II was given 2 gr gel with % 1 phenytoin and Group III was given 2gr gel with %0.1 capsaicin and pure gel. Capsaicin application were used twice a day on 2 consecutive days,subsequently Group III was given only gel on 5 consecutive days. Phenytoin and placebo application were used twice a day on 7 consecutive days. Images were transferred to a computer and ratio of flap necrosis area to total flap area was calculated RESULT: The mean percentage of necrosis in the flaps were 37.27±3.86%, 36.3±6.2%, 23.4±5.9 % in the control, phenytoin and capsaicin groups, respectively. The percentage of flap necrosis was significantly lower in the Capsaicin Group compared to the control group (37.27% vs 23.4%, p<0.01). Although phenytoin had a lower mean percentage of flap necrosins than the control group, this difference was not significant (37.27 vs 36.3 , p>0.05).
We showed topical capsaicin increased the random pattern skin flap survival in rats whereas topical phenytoin had no positive effect. We believe that further studies are required to investigate the efficiency of topical phenytoin applications.
Capsaicin, Phenytoin, Skin flaps.
我们研究了局部应用苯妥英钠和辣椒素对大鼠随意型背部皮瓣的疗效。
本研究使用了21只Wistar大鼠。所有大鼠均掀起3 cm×10 cm的随意型麦克法兰背部皮瓣。在皮瓣与其供区之间放置一个塑料屏障。用4-0尼龙缝线将皮瓣缝合回原位。将大鼠随机分为三组（n = 7）。第一组仅给予凝胶，第二组给予含1%苯妥英钠的2克凝胶，第三组给予含0.1%辣椒素的2克凝胶和纯凝胶。连续2天每天应用辣椒素2次，随后第三组连续5天仅给予凝胶。连续7天每天应用苯妥英钠和安慰剂2次。将图像传输至计算机并计算皮瓣坏死面积与皮瓣总面积的比值。
对照组、苯妥英钠组和辣椒素组皮瓣坏死的平均百分比分别为37.27±3.86%、36.3±6.2%、23.4±5.9%。与对照组相比，辣椒素组皮瓣坏死百分比显著降低（37.27%对23.4%，p<0.01）。虽然苯妥英钠组皮瓣坏死的平均百分比低于对照组，但差异不显著（37.27对36.3，p>0.05）。
我们发现局部应用辣椒素可提高大鼠随意型皮瓣的存活率，而局部应用苯妥英钠无积极作用。我们认为需要进一步研究来探讨局部应用苯妥英钠的有效性。
辣椒素；苯妥英钠；皮瓣
