Bassleer C, Henrotin Y, Franchimont P
Laboratory of Radioimmunology, University of Liège, Belgium.
Scand J Rheumatol Suppl. 1990;81:13-20. doi: 10.3109/03009749009096940.
Human articular chondrocytes may be cultured in three dimensions, according to a method already validated. This model allows us to study the repair processes of the cartilage, by measuring the proliferative activity of chondrocytes and the synthesis of two major constituents of matrix: proteoglycans and type II collagen. Some substances are characterised by stimulatory effect on DNA synthesis and no effect or a defective effect on matrix components: this is the case for Epidermal Growth Factor. Others are able to stimulate (hGH) or to depress (acetyl salicylic acid) both chondrocyte proliferation and matrix components synthesis. Finally, some substances called "chondroprotective", such as the glycosaminoglycan-peptide complex, GP-C (Rumalon) stimulate either the proliferative response or the synthesis of proteoglycans and type II collagen, according to the dose.
根据已验证的方法，人关节软骨细胞可以在三维空间中培养。该模型使我们能够通过测量软骨细胞的增殖活性以及基质的两种主要成分：蛋白聚糖和II型胶原蛋白的合成，来研究软骨的修复过程。某些物质的特征在于对DNA合成有刺激作用，而对基质成分无作用或有缺陷作用：表皮生长因子就是这种情况。其他物质能够刺激（生长激素）或抑制（乙酰水杨酸）软骨细胞增殖和基质成分合成。最后，一些被称为“软骨保护剂”的物质，如糖胺聚糖-肽复合物GP-C（鲁马龙），根据剂量不同，可刺激增殖反应或蛋白聚糖和II型胶原蛋白的合成。
