Franchimont P, Bassleer C, Henrotin Y
Department of Rheumatology, Physical Medicine and Rehabilitation, University of Liege, Belgium.
J Rheumatol Suppl. 1989 Aug;18:5-9.
When cartilage is attacked, either by an excess of charges or by biochemical agents, its morphological and functional impairment is associated with a local homeostatic reaction; this includes a proliferative response (assessed by 3H-thymidine incorporation) and a stimulation of proteoglycan and type II collagen (II coll) synthesis. This homeostatic reaction may be studied in vitro using tridimensional culture of human chondrocytes. Cartilage clusters are formed after 4 days' culture and chondrocytes multiply for the first 15 days of culture. Furthermore, human cartilage proteoglycans and II coll, assayed by specific radioimmunoassays, are released into the culture medium and constitute the new matrix of the clusters. Moreover, it appears that these human chondrocytes are targets for several hormones capable of stimulating a proliferative response without affecting proteoglycan and II coll synthesis.
当软骨受到攻击时，无论是受到过量电荷还是生化因子的攻击，其形态和功能损伤都与局部稳态反应相关；这包括增殖反应（通过3H-胸腺嘧啶核苷掺入评估）以及蛋白聚糖和II型胶原（II coll）合成的刺激。这种稳态反应可以在体外使用人软骨细胞的三维培养进行研究。培养4天后形成软骨簇，软骨细胞在培养的前15天内增殖。此外，通过特异性放射免疫测定法测定的人软骨蛋白聚糖和II coll被释放到培养基中，并构成簇的新基质。此外，似乎这些人软骨细胞是几种激素的靶标，这些激素能够刺激增殖反应而不影响蛋白聚糖和II coll的合成。
