Endodontics, Araçatuba Dental School, UNESP-Univ Estadual Paulista, R. José Bonifácio, Araçatuba, 1193, São Paulo, Brazil.
Clin Oral Investig. 2013 Jul;17(6):1595-9. doi: 10.1007/s00784-012-0853-7. Epub 2012 Oct 5.
The aim of this study was to evaluate triglyceride and cholesterol levels in diabetic rats and their relationship with pulpal and periodontal diseases.
Eighty male rats (Rattus norvegicus albinus, Wistar) were divided into the following eight groups comprising ten animals each: normal rats (G1), rats with pulpal diseases (G2), rats with periodontal diseases (G3), rats with both pulpal and periodontal diseases (G4), diabetic rats (G5), diabetic rats with pulpal diseases (G6), diabetic rats with periodontal diseases (G7), and diabetic rats with both periodontal and pulpal diseases (G8). Diabetes was induced by injecting streptozotocin, periapical lesions were induced by exposing pulpal tissue to the oral environment, and periodontal diseases were induced by periodontal ligature. The animals were killed after 30 days, and lipid profile was enzymatically measured using Trinder's method. The total assessed values were statistically analyzed by analysis of variance and Tukey test (p < 0.05).
The triglyceride levels of diabetic rats with periodontal disease and of diabetic rats with both periodontal and pulpal diseases were significantly higher than those of normal rats and nondiabetic group rats, respectively. The differences in the cholesterol levels among the groups were not significant.
We found that the association of pulpal and periodontal diseases with diabetes increased triglyceride levels in rats.
Changes in lipid profile may be related to the presence of oral infections and diabetes.
本研究旨在评估糖尿病大鼠的甘油三酯和胆固醇水平及其与牙髓和牙周疾病的关系。
将 80 只雄性大鼠（白化 Wistar 大鼠）分为以下 8 组，每组 10 只：正常大鼠（G1）、牙髓疾病大鼠（G2）、牙周疾病大鼠（G3）、牙髓和牙周疾病大鼠（G4）、糖尿病大鼠（G5）、牙髓疾病糖尿病大鼠（G6）、牙周疾病糖尿病大鼠（G7）和牙髓和牙周疾病糖尿病大鼠（G8）。通过注射链脲佐菌素诱导糖尿病，通过将牙髓组织暴露于口腔环境中来诱导根尖周病变，通过牙周韧带诱导牙周疾病。30 天后处死动物，使用 Trinder 法通过酶法测量脂质谱。通过方差分析和 Tukey 检验（p<0.05）对总评估值进行统计学分析。
患有牙周病的糖尿病大鼠和同时患有牙周病和牙髓病的糖尿病大鼠的甘油三酯水平明显高于正常大鼠和非糖尿病组大鼠。各组之间的胆固醇水平差异无统计学意义。
我们发现，牙髓和牙周疾病与糖尿病的联合增加了大鼠的甘油三酯水平。
脂质谱的变化可能与口腔感染和糖尿病的存在有关。
