Department of Endodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, Brazil.
Int Endod J. 2014 Mar;47(3):228-37. doi: 10.1111/iej.12136. Epub 2013 Jun 12.
To measure glycosylated haemoglobin (HbA1c) in a diabetic model as a means of investigating apical periodontitis and periodontal disease for their effects on both blood glucose concentrations and long-term glycaemic control.
Wistar rats (n = 80) were assigned to one of eight groups (10 animals/group): control (G1), apical periodontitis (G2), periodontal disease (G3), apical periodontitis and periodontal disease (G4), diabetic (G5), diabetic with apical periodontitis (G6), diabetic with periodontal disease (G7) and diabetic with apical periodontitis and periodontal disease (G8). A diabetic state was induced with streptozotocin. Apical periodontitis was induced by dental exposure to the oral environment. Periodontal disease was induced by periodontal ligature. Blood glucose concentrations were measured at 0, 6, 30 and 60 days. After euthanization, rat maxillae were excised and processed for histopathology and for measurement of HbA1c levels by ion exchange chromatography. Data were tabulated and subject to statistical analysis (P < 0.05).
The inflammatory infiltrate and alveolar bone resorption were more severe in diabetic rats (P < 0.05). Diabetic rats exhibited higher levels of HbA1c independent of apical periodontitis or periodontal disease (P < 0.05). However, the presence of oral infections in diabetic rats was associated with increased blood glucose concentrations (P < 0.05).
Oral infections affect glycaemic conditions in diabetic rats and increase HbA1c levels in normoglycaemic or diabetic rats.
通过测量糖尿病模型中的糖化血红蛋白（HbA1c），研究根尖周炎和牙周病对血糖浓度和长期血糖控制的影响。
将 Wistar 大鼠（n=80）分为 8 组（每组 10 只）：对照组（G1）、根尖周炎组（G2）、牙周病组（G3）、根尖周炎和牙周病组（G4）、糖尿病组（G5）、糖尿病伴根尖周炎组（G6）、糖尿病伴牙周病组（G7）和糖尿病伴根尖周炎和牙周病组（G8）。用链脲佐菌素诱导糖尿病状态。通过暴露于口腔环境中的牙齿来诱导根尖周炎。通过牙周结扎来诱导牙周病。在 0、6、30 和 60 天测量血糖浓度。安乐死后，取出大鼠上颌骨进行组织病理学检查，并通过离子交换色谱法测量 HbA1c 水平。将数据制表并进行统计分析（P<0.05）。
糖尿病大鼠的炎症浸润和牙槽骨吸收更为严重（P<0.05）。无论是否存在根尖周炎或牙周病，糖尿病大鼠的 HbA1c 水平均较高（P<0.05）。然而，糖尿病大鼠口腔感染的存在与血糖浓度升高有关（P<0.05）。
口腔感染影响糖尿病大鼠的血糖状况，并增加正常血糖或糖尿病大鼠的 HbA1c 水平。
