利用金属增强荧光法对临床低蛋白血症样本中的人血清白蛋白进行高灵敏度定量分析。

Highly sensitive quantitation of human serum albumin in clinical samples for hypoproteinemia using metal-enhanced fluorescence.

机构信息

Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 701 East Pratt St, Baltimore, MD 21202, USA.

出版信息

J Fluoresc. 2013 Jan;23(1):187-92. doi: 10.1007/s10895-012-1133-2. Epub 2012 Oct 11.

DOI:10.1007/s10895-012-1133-2

PMID:23054300

Abstract

In this paper, we have explored metal-enhanced fluorescence (MEF) of the Human serum albumin indicators: Albumin Blue 580, Merocyanine 540 and Bromophenol Blue in close proximity to silver nano-particles, SiFs, from both buffered and clinical samples. The photostability of the Albumin Blue 580 is shown to be much more prolonged from the SiFs as compared to glass (a control sample), potentially allowing for longer detection times to further improve assay statistics. Our findings suggest the widespread use of nanoparticulate SiFs surfaces for the enhanced detection of HSA, particularly for Hypoproteinemia, where an enhanced assay performance at low protein abundance is required.

摘要

在本文中，我们探讨了人血清白蛋白指示剂（Albumin Blue 580、Merocyanine 540 和 Bromophenol Blue）在接近银纳米粒子（SiFs）时的金属增强荧光（MEF），这些 SiFs 来自缓冲液和临床样本。与玻璃（对照样品）相比，Albumin Blue 580 的光稳定性在 SiFs 中得到了显著延长，这可能允许更长的检测时间以进一步提高分析统计的准确性。我们的研究结果表明，纳米颗粒 SiFs 表面可广泛用于增强人血清白蛋白的检测，特别是在低蛋白丰度下需要增强分析性能的低蛋白血症中。