Aslan Kadir, Leonenko Zoya, Lakowicz Joseph R, Geddes Chris D
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, USA.
J Phys Chem B. 2005 Mar 3;109(8):3157-62. doi: 10.1021/jp045186t.
Two methods have been considered for the deposition of silver nanorods onto conventional glass substrates. In the first method, silver nanorods were deposited onto 3-(aminopropyl)triethoxysilane-coated glass substrates simply by immersing the substrates into the silver nanorod solution. In the second method, spherical silver seeds that were chemically attached to the surface were subsequently converted and grown into silver nanorods in the presence of a cationic surfactant and silver ions. The size of the silver nanorods was controlled by sequential immersion of silver seed-coated glass substrates into a growth solution and by the duration of immersion, ranging from tens of nanometers to a few micrometers. Atomic force microscopy and optical density measurements were used to characterize the silver nanorods deposited onto the surface of the glass substrates. The application of these new surfaces is for metal-enhanced fluorescence (MEF), whereby the close proximity of silver nanostructures can alter the radiative decay rate of fluorophores, producing enhanced signal intensities and an increased fluorophore photostability. In this paper, it is indeed shown that irregularly shaped silver nanorod-coated surfaces are much better MEF surfaces as compared to traditional silver island or colloid films. Subsequently, these new silver nanorod preparation procedures are likely to find a common place in MEF, as they are a quicker and much cheaper alternative as compared to surfaces fabricated by traditional nanolithographic techniques.
已经考虑了两种将银纳米棒沉积到传统玻璃基板上的方法。在第一种方法中，通过将基板浸入银纳米棒溶液中，将银纳米棒简单地沉积到3-(氨丙基)三乙氧基硅烷涂层的玻璃基板上。在第二种方法中，化学附着在表面的球形银种子随后在阳离子表面活性剂和银离子存在下转化并生长成银纳米棒。银纳米棒的尺寸通过将涂有银种子的玻璃基板依次浸入生长溶液中以及浸入持续时间来控制，范围从几十纳米到几微米。原子力显微镜和光密度测量用于表征沉积在玻璃基板表面的银纳米棒。这些新表面的应用是用于金属增强荧光(MEF)，由此银纳米结构的紧密接近可以改变荧光团的辐射衰减率，产生增强的信号强度和增加的荧光团光稳定性。在本文中，确实表明与传统的银岛或胶体膜相比，形状不规则的银纳米棒涂层表面是更好的MEF表面。随后，这些新的银纳米棒制备程序可能会在MEF中找到一席之地，因为与通过传统纳米光刻技术制造的表面相比，它们是一种更快且更便宜的替代方案。
