Department of Pharmacology and Toxicology, Hospital Infántil de México Federico Gómez (HIMFG), Mexico City, Mexico.
Endocrine. 2013 Jun;43(3):603-10. doi: 10.1007/s12020-012-9808-7. Epub 2012 Sep 29.
The aim of this study was to evaluate the effect of a six-month lifestyle intervention on ghrelin and asymmetrical dimethylarginine (ADMA) in obese Mexican adolescents. A total of 65 obese Mexican adolescents aged 10-16 years completed a six-month lifestyle intervention. Anthropometric and biochemical parameters were assessed at baseline and at six months. Twenty normal-weight adolescents were also evaluated at baseline. Insulin resistance (IR) was determined by the homeostasis model assessment of IR (HOMA-IR). Ghrelin and ADMA were determined by enzyme-linked immunosorbent assay. Obese adolescents presented significantly higher triglycerides, cholesterol, glucose, insulin, HOMA-IR, and ADMA levels, while ghrelin was significantly lower. The lifestyle intervention led to a significant improvement in HOMA-IR, ghrelin, and ADMA in the whole studied obese subjects. ADMA and ghrelin levels were associated with BMI and IR components. According to the value of HOMA-IR, the obese subjects were divided into subjects with or without IR, no difference in ghrelin and ADMA was observed in these two subgroups. After intervention, the obese with IR showed increased ghrelin and decreased ADMA, while the obese without IR only showed improvement in ghrelin. The multiple linear regression analysis revealed that the changes of systolic blood pressure were the only predictor for the changes of ghrelin in the obese with IR. Our study demonstrated the increase of ADMA and the decrease of ghrelin in obese adolescents. Lifestyle intervention improved insulin resistance, decreased ADMA, and increased ghrelin in obese subjects with IR although no significant weight loss was observed.
本研究旨在评估为期六个月的生活方式干预对肥胖墨西哥青少年生长激素释放肽（ghrelin）和非对称性二甲基精氨酸（ADMA）的影响。共有 65 名肥胖的墨西哥青少年（年龄 10-16 岁）完成了为期六个月的生活方式干预。在基线和六个月时评估了人体测量和生化参数。还在基线时评估了 20 名正常体重的青少年。胰岛素抵抗（IR）通过稳态模型评估的胰岛素抵抗（HOMA-IR）来确定。通过酶联免疫吸附试验测定生长激素释放肽和 ADMA。肥胖青少年的甘油三酯、胆固醇、葡萄糖、胰岛素、HOMA-IR 和 ADMA 水平显著升高，而生长激素释放肽水平显著降低。生活方式干预使所有研究的肥胖受试者的 HOMA-IR、生长激素释放肽和 ADMA 均显著改善。ADMA 和生长激素释放肽水平与 BMI 和 IR 成分有关。根据 HOMA-IR 的值，将肥胖受试者分为存在或不存在 IR 的两组，在这两组中，生长激素释放肽和 ADMA 没有差异。干预后，IR 肥胖者的生长激素释放肽增加，ADMA 减少，而无 IR 肥胖者仅表现出生长激素释放肽的改善。多元线性回归分析显示，IR 肥胖者的收缩压变化是生长激素释放肽变化的唯一预测因子。我们的研究表明，肥胖青少年的 ADMA 增加和生长激素释放肽减少。生活方式干预改善了肥胖伴 IR 患者的胰岛素抵抗，降低了 ADMA，增加了生长激素释放肽，尽管未观察到明显的体重减轻。
