利用荧光 D-氨基酸原位探测活细菌中新合成的肽聚糖。
In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
机构信息
Indiana University, Bloomington, IN 47405, USA.
出版信息
Angew Chem Int Ed Engl. 2012 Dec 7;51(50):12519-23. doi: 10.1002/anie.201206749. Epub 2012 Oct 10.
Abstract
Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.
摘要
追踪细菌的生命轨迹
通过利用细胞对不同非天然 D-氨基酸的耐受性，对不同细菌的肽聚糖（PG）进行标记。这些无毒的 D-氨基酸优先标记 PG 合成的活性位点，从而能够对系统发生和形态上多样的细菌的细胞壁动态进行精细的时空追踪。HCC = 7-羟基香豆素，NBD = 7-硝基苯并呋咱，TAMRA = 羧基四甲基罗丹明。
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