Dolo A, Coulibaly M, Maïga B, Daou M, Arama C, Troye-Blomberg M, Doumbo O
Malaria Research and Training Center/département d'épidémiologie des affections parasitaires, de pharmacie et d'odontostomatologie (FMPOS), Bamako, Mali.
Bull Soc Pathol Exot. 2012 Dec;105(5):364-9. doi: 10.1007/s13149-012-0262-9. Epub 2012 Oct 10.
Fulani of Mali are known for their lower susceptibility to Plasmodium falciparum malaria than their neighbours, the Dogon, despite similar transmission conditions. However, the mechanisms underlying these differences are poorly understood, particularly those concerning antigenspecific immune responses. The Apical Membrane Antigen 1 (AMA1) and the Merozoite Surface Antigen 1 (MSP1) are two malaria vaccine candidates, which play a pivotal role during the invasion of parasites into erythrocytes, and in the case of AMA1, of hepatocytes. Therefore, we analyzed the level of anti-AMA1 and anti-MSP1 antibodies (FVO and 3D7 alleles), by using ELISA (Enzyme Linked Immuno Sorbent Assay) to investigate whether there are differences between the two ethnic groups. Our results show that the splenic rate, the level of anti-AMA1 and anti-MSP1 were significantly higher in Fulani compared to Dogon; while the parasite rate was lower in Fulani group compared to Dogon. Our results suggest that the lower susceptibility of Fulani to malaria could be due to the higher specific humoral responses against AMA1 and MSP 1 in Fulani's ethnic group compared to Dogon.
马里的富拉尼人以对恶性疟原虫疟疾的易感性低于其邻居多贡人而闻名，尽管传播条件相似。然而，这些差异背后的机制尚不清楚，尤其是那些与抗原特异性免疫反应有关的机制。顶端膜抗原1（AMA1）和裂殖子表面抗原1（MSP1）是两种疟疾疫苗候选物，它们在寄生虫侵入红细胞的过程中起着关键作用，就AMA1而言，还在侵入肝细胞的过程中起关键作用。因此，我们通过酶联免疫吸附测定（ELISA）分析了抗AMA1和抗MSP1抗体（FVO和3D7等位基因）的水平，以研究这两个族群之间是否存在差异。我们的结果表明，与多贡人相比，富拉尼人的脾脏比率、抗AMA1和抗MSP1水平显著更高；而富拉尼组的寄生虫比率比多贡人低。我们的结果表明，富拉尼人对疟疾的易感性较低可能是由于与多贡人相比，富拉尼族群对AMA1和MSP1具有更高的特异性体液反应。
