Lied Gülen Arslan, Milde Anne Marita, Nylund Kim, Mujic Maja, Grimstad Tore, Hausken Trygve, Gilja Odd Helge
Institute of Medicine, University of Bergen, Norway.
Clin Exp Gastroenterol. 2012;5:195-201. doi: 10.2147/CEG.S31150. Epub 2012 Oct 2.
Experimentally induced colitis is used in animals to investigate pathophysiological mechanisms in inflammatory bowel disease. When following disease course and treatment effects, it should be possible to perform repeated measurements without harming the animals. This pilot study was performed to investigate whether transabdominal ultrasound using a clinical scanner could be used on rats to demonstrate bowel inflammation in an experimental colitis model. Colitis was induced by either 5% dextran sodium sulfate (DSS) in drinking water for 7 days or a single dose of intracolonic trinitrobenzene sulfonic acid (TNBS). Using ultrasonography, wall thickness of distal colon, cecum, and small bowel was recorded prior to and after DSS, and prior to, 2, and 7 days after TNBS. Blood (tumor necrosis factor [TNF]-alpha) and fecal samples (HemoFEC occult blood) were taken from each group on the same days as sonography. Thereafter, rats were killed and specimens for histology were taken. Wall thickness of distal colon, not of cecum or small bowel, increased significantly after 7 days of DSS, and wall thickness of both distal colon and small bowel increased on day 2 and 7 after TNBS. TNF-alpha increased after 7 days in the latter group only. There was a significant correlation between ultrasonographic measurements and combined histology score of distal colon in the DSS group. HemoFEC was also positive in accordance with sonographic and histological features. Increased intestinal wall thickness in response to both DSS- and TNBS-induced colitis was able to be visualized by transabdominal sonography. Moreover, ultrasound findings, occult blood sampling, and histological findings supported each other, indicating that ultrasonography can be used to assess inflammation in a rat experimental model.
在动物身上诱导实验性结肠炎，以研究炎症性肠病的病理生理机制。在跟踪疾病进程和治疗效果时，应该能够在不伤害动物的情况下进行重复测量。本初步研究旨在调查使用临床扫描仪进行经腹超声检查是否可用于大鼠，以在实验性结肠炎模型中显示肠道炎症。通过在饮用水中加入5%的葡聚糖硫酸钠（DSS）7天或单次结肠内注射三硝基苯磺酸（TNBS）来诱导结肠炎。使用超声检查，记录DSS处理前后以及TNBS处理前、处理后2天和7天远端结肠、盲肠和小肠的壁厚度。在与超声检查同一天从每组采集血液（肿瘤坏死因子[TNF]-α）和粪便样本（HemoFEC潜血）。此后，处死大鼠并采集用于组织学检查的标本。DSS处理7天后，远端结肠而非盲肠或小肠的壁厚度显著增加，TNBS处理后第2天和第7天，远端结肠和小肠的壁厚度均增加。仅在后一组中，TNF-α在7天后升高。在DSS组中，超声测量与远端结肠的组织学综合评分之间存在显著相关性。HemoFEC也根据超声和组织学特征呈阳性。经腹超声能够显示DSS和TNBS诱导的结肠炎导致的肠壁厚度增加。此外，超声检查结果、潜血采样和组织学检查结果相互支持，表明超声检查可用于评估大鼠实验模型中的炎症。
