He Linwen, Huang Aiyou, Shen Songdong, Niu Jianfeng, Wang Guangce
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences (IOCAS), Nanhai Road 7, Qingdao 266071, China ; School of Earth Science, Graduate University of Chinese Academy of Sciences, Yuquan Road 19, Beijing 100049, China.
Comp Funct Genomics. 2012;2012:912843. doi: 10.1155/2012/912843. Epub 2012 Sep 26.
Porphyra yezoensis Ueda is an intertidal marine red algae that has received increasing attention as a model organism owing to its important role in biological research and the agronomic industry. The two generations of Porphyra yezoensis, the sporophyte and the gametophyte, have the same genome but show great differences in many aspects, including structural features, habitat, and gene expression. To identify miRNAs and their probable roles in P. yezoensis development, we constructed and sequenced libraries of small RNA from P. yezoensis sporophytes and gametophytes. The sequencing data were analyzed, and 14 miRNAs were identified, with only one common to these two samples. Our results show that P. yezoensis has a complex small RNA processing system containing novel miRNAs that have no identifiable homolog in other organisms. These miRNAs might have important regulatory roles in development of the different generations of P. yezoensis.
条斑紫菜是一种潮间带海洋红藻，由于其在生物学研究和农业产业中的重要作用，作为一种模式生物受到了越来越多的关注。条斑紫菜的两个世代，即孢子体和配子体，具有相同的基因组，但在许多方面表现出很大差异，包括结构特征、栖息地和基因表达。为了鉴定微小RNA（miRNA）及其在条斑紫菜发育中可能的作用，我们构建了条斑紫菜孢子体和配子体的小RNA文库并进行测序。对测序数据进行分析后，鉴定出14个miRNA，这两个样本中只有一个是共同的。我们的结果表明，条斑紫菜具有一个复杂的小RNA加工系统，其中包含在其他生物中没有可识别同源物的新型miRNA。这些miRNA可能在条斑紫菜不同世代的发育中具有重要的调控作用。
