Casslén B G, Siler-Khodr T M, Harper M J
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio.
Acta Endocrinol (Copenh). 1990 Jan;122(1):137-44. doi: 10.1530/acta.0.1220137.
Prolactin was produced by endometrial stromal cells, but not by epithelial cells, in primary culture. Production was stimulated by progesterone in a time and dose (1-1000 nmol/l) dependent way. Stimulation was started one day after plating. The release of prolactin started one day earlier in cultures from luteal phase than proliferative phase endometria. The responsiveness to progesterone declined with time in culture, sooner in luteal than in proliferative phase cultures, but could, at least partly, be maintained by including estradiol (10 nmol/l) in the medium. Lost response to progesterone could be restored by stimulation with estradiol for two days. The results indicate that prolactin synthesis in the endometrium is regulated directly by progesterone, and indirectly by estradiol. We suggest that prolactin production in primary stromal cell cultures could serve as a bioassay for progestational activity of steroid hormones in the human endometrium.
在原代培养中，催乳素由子宫内膜基质细胞产生，而非上皮细胞。孕酮以时间和剂量（1 - 1000 nmol/L）依赖的方式刺激其产生。接种一天后开始刺激。黄体期子宫内膜培养物中催乳素的释放比增殖期子宫内膜培养物早一天开始。随着培养时间的推移，对孕酮的反应性下降，黄体期培养物比增殖期培养物下降得更快，但通过在培养基中加入雌二醇（10 nmol/L），反应性至少可以部分维持。用雌二醇刺激两天可恢复对孕酮丧失的反应。结果表明，子宫内膜中催乳素的合成直接受孕酮调节，间接受雌二醇调节。我们认为，原代基质细胞培养中催乳素的产生可作为人类子宫内膜中甾体激素孕激素活性的生物测定方法。
