Ying Y K, Walters C A, Kuslis S, Lin J T, Daly D C, Riddick D H
Am J Obstet Gynecol. 1985 Mar 15;151(6):801-4. doi: 10.1016/0002-9378(85)90524-1.
The production of prolactin by explants of late secretory endometrium has been correlated with the extent of decidual differentiation. This correlation is strengthened by the observation that luteal phase defective endometrium produces less prolactin than normal control endometrium in a 24-hour in vitro culture system. In the present study the prolactin production by explants of normal, luteal phase defective, progesterone-corrected luteal phase defective, and clomiphene- or follicle-stimulating hormone/luteinizing hormone-corrected luteal phase defective late secretory endometrium was measured over 96 hours at 24-hour intervals. Progesterone in physiologic concentrations was added to the culture medium to maintain tissue integrity and prolactin synthesis. The prolactin production of normal late secretory endometrium rose over 96 hours under progesterone stimulation. The luteal phase defective endometrium produced significantly less prolactin under the same conditions. Histologically proven corrected luteal phase defective endometrium, regardless of treatment method, produced prolactin not different from the normal controls of the same dates. From these results it is concluded that histologic correction of luteal phase defective endometrium is associated with a corresponding biochemical correction with use of prolactin as a metabolic marker. The findings also strongly support timed endometrial biopsy as the method of diagnosis and evaluation of treatment of luteal phase defect.
分泌期晚期子宫内膜外植体分泌催乳素的情况与蜕膜分化程度相关。在24小时体外培养系统中观察到黄体期缺陷型子宫内膜产生的催乳素比正常对照子宫内膜少，这一相关性得到了加强。在本研究中，对正常、黄体期缺陷型、孕激素校正后的黄体期缺陷型以及克罗米芬或促卵泡激素/促黄体生成素校正后的黄体期缺陷型分泌期晚期子宫内膜外植体在96小时内每隔24小时测量一次催乳素分泌量。向培养基中添加生理浓度的孕激素以维持组织完整性和催乳素合成。在孕激素刺激下，正常分泌期晚期子宫内膜的催乳素分泌量在96小时内有所增加。在相同条件下，黄体期缺陷型子宫内膜产生的催乳素明显较少。经组织学证实的校正后的黄体期缺陷型子宫内膜，无论采用何种治疗方法，其分泌的催乳素与同期正常对照无差异。从这些结果可以得出结论，黄体期缺陷型子宫内膜的组织学校正与使用催乳素作为代谢标志物的相应生化校正相关。这些发现也有力地支持了定时子宫内膜活检作为诊断和评估黄体期缺陷治疗方法的依据。
