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LecB 与铜绿假单胞菌表面的特定关联：外膜蛋白 OprF 的作用。

Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.

机构信息

Institute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Juelich, Germany.

出版信息

PLoS One. 2012;7(10):e46857. doi: 10.1371/journal.pone.0046857. Epub 2012 Oct 8.

DOI:10.1371/journal.pone.0046857
PMID:23056489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3466170/
Abstract

The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.

摘要

岩藻糖结合凝集素 LecB 影响生物膜形成并参与铜绿假单胞菌的致病性。LecB 位于外膜上，并且可以通过用岩藻糖处理外膜部分而特异性释放，表明它结合特定的配体。在这里，我们报告 LecB 结合到外膜蛋白 OprF。在 OprF 缺陷的铜绿假单胞菌突变体中，LecB 不再可检测到在膜中，而是在培养上清液中，表明 LecB 和 OprF 之间存在特异性相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/373deba15305/pone.0046857.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/bce2d5997245/pone.0046857.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/5dd735658fd1/pone.0046857.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/cf9f52ce1a03/pone.0046857.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/373deba15305/pone.0046857.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/bce2d5997245/pone.0046857.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/5dd735658fd1/pone.0046857.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/cf9f52ce1a03/pone.0046857.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22a9/3466170/373deba15305/pone.0046857.g004.jpg

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