Liu Chia-Hsing, Lin Chih-Hung, Tsai Min-Jan, Chen Wan-Tzu, Chai Chee-Yin, Huang Ya-Chun, Tsai Kun-Bow
Department of Pathology, Kaohsiung Medical University Hospital.
Appl Immunohistochem Mol Morphol. 2013 May;21(3):275-9. doi: 10.1097/PAI.0b013e31826d81db.
Melanins are naturally occurring pigments in both normal and pathologic tissues. Two common bleaching processes are potassium permanganate followed by oxalic acid treatment and dilute hydrogen peroxide (H2O2) process. The potassium permanganate/oxalic acid method is faster and more easily incorporated in conventional daily immunostaining protocols, whereas the dilute H2O2 method requires 24 hours. This study aimed to reduce melanin bleaching time by using a 10% H2O2 dilution. First, reaction time was reduced to 30 minutes by raising the temperature to 65°C. Second, containers with high thermal conductivity were used to improve bleaching effectiveness. Experimental comparisons of melanin treatments with H2O2 contained in an iron jar, a glass coplin jar, and a plastic steel jar obtained bleaching time of 20, 30, and 40 minutes, respectively. These modifications of the conventional bleaching method significantly improve the speed and efficiency of the procedure and are recommended when performing immunohistochemical studies.
黑色素是正常组织和病理组织中天然存在的色素。两种常见的漂白方法是高锰酸钾后接草酸处理以及稀过氧化氢（H2O2）法。高锰酸钾/草酸法速度更快，更易于纳入常规日常免疫染色方案中，而稀H2O2法需要24小时。本研究旨在通过使用10%的H2O2稀释液来减少黑色素漂白时间。首先，将温度提高到65°C，使反应时间缩短至30分钟。其次，使用具有高导热性的容器来提高漂白效果。分别在铁罐、玻璃科普林瓶和塑钢瓶中用H2O2对黑色素进行处理的实验比较，得到的漂白时间分别为20分钟、30分钟和40分钟。对传统漂白方法的这些改进显著提高了该程序的速度和效率，在进行免疫组织化学研究时推荐使用。
