Liu Chia-Hsing, Lin Chih-Hung, Tsai Min-Jan, Chen Yu-Hsuan, Yang Sheau-Fang, Tsai Kun-Bow
1 Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
2 Kaohsiung Municipal Hsiaokang Hospital, Kaohsiung, Taiwan.
Int J Surg Pathol. 2018 Aug;26(5):410-416. doi: 10.1177/1066896918756998. Epub 2018 Feb 21.
Diagnosing melanocytic lesions is among the most challenging problems in the practice of pathology. The difficulty of physically masking melanin pigment and the similarity of its color to commonly used chromogens often complicate examination of the cytomorphology and immunohistochemical staining results for tumor cells. Melanin bleach can be very helpful for histopathological diagnosis of heavily pigmented melanocytic lesions. Although various depigmentation methods have been reported, no standardized methods have been developed. This study developed a fully automated platform that incorporates hydrogen peroxide-based melanin depigmentation in an automated immunohistochemical analysis.
The utility of the method was tested in 1 cell block of malignant melanoma cells in pleural effusion, 10 ocular melanoma tissue samples, and 10 cutaneous melanoma tissue samples. Our results demonstrated that the proposed method, which can be performed in only 3 hours, effectively preserves cell cytomorphology and immunoreactivity.
The method is particularly effective for removing melanin pigment to facilitate histopathological examination of cytomorphology and for obtaining an unmasked tissue section for immunohistochemical analysis.
诊断黑素细胞性病变是病理学实践中最具挑战性的问题之一。物理遮盖黑色素的难度以及其颜色与常用显色剂的相似性，常常使肿瘤细胞的细胞形态学检查和免疫组织化学染色结果变得复杂。黑色素漂白对于色素沉着严重的黑素细胞性病变的组织病理学诊断非常有帮助。尽管已经报道了各种脱色方法，但尚未开发出标准化方法。本研究开发了一个全自动平台，该平台在自动免疫组织化学分析中结合了基于过氧化氢的黑色素脱色方法。
该方法在1个胸腔积液中恶性黑色素瘤细胞的细胞块、10个眼黑色素瘤组织样本和10个皮肤黑色素瘤组织样本中进行了测试。我们的结果表明，所提出的方法仅需3小时即可完成，能有效保留细胞形态和免疫反应性。
该方法在去除黑色素以促进细胞形态学的组织病理学检查以及获得用于免疫组织化学分析的未遮盖组织切片方面特别有效。
