Weon Jin Bae, Lee Bohyoung, Yun Bo-Ra, Lee Jiwoo, Ma Choong Je
Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Pharmacogn Mag. 2012 Jul;8(31):231-6. doi: 10.4103/0973-1296.99289.
Cynanchum paniculatum Kitagawa belongs to Asclepiadaceae and was used in traditional medicine to invigorate blood, alleviate edema, relieve pain, and relieve toxicity for a long time.
A novel and reliable high performance liquid chromatography coupled with diode array detector method has been established for simultaneous determination of 10 bioactive compounds isolated from Cynanchum paniculatum Kitagawa, one of the herbal medicines.
The chromatography analysis was performed on a SHISEIDO C(18) column (S-5 μm, 4.6 mm I.D. × 250 mm) at 35°C with a gradient elution of acetonitrile and water at a flow rate of 1ml/min and UV detection at 210, 230, and 280 nm.
The method was validated for linearity, precision, and accuracy. All calibration curves showed good linear regression (r(2) > 0.9996). Limits of detection (LOD) and limits of quantification (LOQ) fell in the ranges 0.01 - 0.28 μg/ml and 0.04 - 0.83 μg/ml, respectively. The relative standard deviation (RSD) of the intra- and inter- day test, precision test were within 1.92% and 2.43%, respectively. The mean recovery of all ranged from 92.82 to 107.96% with RSD values 0.12 - 2.18%.
The results of validation appeared that this established method was very accurate and stabilized for the quantification of 10 bioactive compounds isolated from C. paniculatum.
白首乌属于萝藦科，长期以来在传统医学中用于活血、利水、止痛、解毒。
建立一种新颖可靠的高效液相色谱-二极管阵列检测法，用于同时测定从草药白首乌中分离出的10种生物活性化合物。
色谱分析在资生堂C18柱（S-5μm，4.6mm内径×250mm）上进行，柱温35℃，以乙腈和水梯度洗脱，流速1ml/min，在210、230和280nm波长处进行紫外检测。
该方法进行了线性、精密度和准确度验证。所有校准曲线均显示出良好的线性回归（r2>0.9996）。检测限（LOD）和定量限（LOQ）分别在0.01 - 0.28μg/ml和0.04 - 0.83μg/ml范围内。日内和日间试验的相对标准偏差（RSD），精密度试验分别在1.92%和2.43%以内。所有的平均回收率在92.82%至107.96%之间，RSD值为0.12 - 2.18%。
验证结果表明，该建立的方法对于定量从白首乌中分离出的10种生物活性化合物非常准确和稳定。
