Weon Jin Bae, Yang Hye Jin, Ma Jin Yeul, Ma Choong Je
Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, South Korea.
Pharmacogn Mag. 2011 Jan;7(25):60-4. doi: 10.4103/0973-1296.75903.
Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity.
For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established.
To develop the method, a reverse phase column, DIONEX C (18) (5 μm, 120 µ, 4.6 mm × 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test.
All calibration curves of components showed good linearity (R (2) > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 μg/ml and 0.04 to 0.53 μg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.
This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.
传统草药防己地黄汤被认为是一种治疗肥胖症的方剂。
建立一种高效液相色谱 - 二极管阵列检测法，用于同时测定防己地黄汤中的五种成分（芍药苷、6 - 姜酚、去甲氧基姜黄素、栀子苷和甘草酸）。
为开发该方法，使用了反相柱DIONEX C(18)（5μm，120μ，4.6mm×150mm）。流动相由甲醇和水组成，采用梯度洗脱。紫外波长设置为230、240和254nm。通过线性、精密度测试和回收率测试完成方法验证。
各成分的校准曲线均显示出良好的线性（R(2)>0.9959）。检测限（LOD）和定量限（LOQ）分别为0.01至0.17μg/ml和0.04至0.53μg/ml。精密度测试的日内和日间测试相对标准偏差（RSD）值分别小于0.43%和1.26%。在回收率测试中，准确度结果为95.27%至107.70%，RSD值小于2.21%。
该开发的方法应用于市售防己地黄汤样品，五种标记成分有效分离，无任何成分峰的干扰。
