Shekarchi M, Hajimehdipoor H, Khanavi M, Adib N, Bozorgi M, Akbari-Adergani B
Food and Drug Control Laboratories and Food and Drug Laboratory Research Center, MOH and ME, Tehran, Iran.
Pharmacogn Mag. 2010 Jan;6(21):13-8. doi: 10.4103/0973-1296.59961. Epub 2010 Feb 13.
Swertia spp. (Gentianaceae) grow widely in the eastern and southern Asian countries and are used as traditional medicine for gastrointestinal disorders. Swerchirin, one of the xanthones in Swertia spp., has many pharmacological properties, such as, antimalarial, antihepatotoxic, and hypoglycemic effects. Because of the pharmacological importance of Swerchirin in this investigation, it was purified from Swertia longifolia Boiss. as one of the main components and quantified by means of a validated high performance liquid chromatography (HPLC) technique. Aerial parts of the plant were extracted with acetone 80%. Phenolic and non-phenolic constituents of the extract were separated from each other during several processes. The phenolic fraction was injected into the semi-preparative HPLC system, which consisted of a C(18) column and a gradient methanol: 0.1% formic acid mode. Using this method, we were able to purify six xanthones from the plant, in order to use them as standard materials. The analytical method was validated for Swerchirin as one of the most important components of the plant, with more pharmacological activities according to the validation parameters, such as, selectivity, linearity (r(2) > 0.9998), precision (</=3.3), and accuracy, which were measured by the determination of recovery (98-107%). The limits of detection and quantization were found to be 2.1 and 6.3 mug/mL, respectively. On account of the speed and accuracy, the UV-HPLC method may be used for quantitative analysis of Swerchirin.
獐牙菜属植物（龙胆科）广泛生长于东亚和南亚国家，被用作治疗胃肠道疾病的传统药物。獐牙菜苦苷是獐牙菜属植物中的一种氧杂蒽酮，具有多种药理特性，如抗疟疾、抗肝毒性和降血糖作用。由于獐牙菜苦苷在本研究中的药理重要性，它作为主要成分之一从长叶獐牙菜中纯化出来，并通过经过验证的高效液相色谱（HPLC）技术进行定量。用80%丙酮提取该植物的地上部分。提取物中的酚类和非酚类成分在几个过程中相互分离。将酚类部分注入半制备HPLC系统，该系统由C(18)柱和甲醇：0.1%甲酸梯度模式组成。使用这种方法，我们能够从该植物中纯化出六种氧杂蒽酮，以便将它们用作标准物质。该分析方法针对獐牙菜苦苷作为该植物最重要的成分之一进行了验证，根据验证参数，如选择性、线性（r(2)>0.9998）、精密度（</=3.3）和准确度，通过回收率测定（98 - 107%）进行测量。检测限和定量限分别为2.1和6.3μg/mL。由于速度和准确性，UV - HPLC方法可用于獐牙菜苦苷的定量分析。
