Ruiz Juan P, Pelaez Daniel, Dias Janice, Ziebarth Noël M, Cheung Herman S
Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, FL, USA ; Research Service and Geriatrics Research, Education, and Clinical Center, Veterans Affairs Medical Center, Miami, FL, USA.
Cell Health Cytoskelet. 2012 Mar 28;4:29-35. doi: 10.2147/CHC.S24381.
To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. METHODS: MSCs were synchronized to the G(0) stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 µM, 0.5 µM, and 1 µM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. RESULTS: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 µM and 1.0 µM concentrations only. CONCLUSIONS: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.
测量人间充质干细胞（MSCs）细胞核与细胞质的弹性以及体外暴露于尼古丁所引起的变化。方法：通过血清剥夺技术将MSCs同步至细胞周期的G(0)期。然后用含有尼古丁（0.1 μM、0.5 μM和1 μM）的培养基处理细胞。接着使用原子力显微镜测量这些细胞的细胞核与细胞质的杨氏模量。结果：对于未同步化和同步化的细胞，细胞核均比细胞质软，尽管未发现这种差异具有统计学意义。对于所有浓度，用尼古丁处理的细胞的细胞核明显比对照硬。仅在0.5 μM和1.0 μM浓度下，尼古丁处理的细胞的细胞质比对照细胞明显更硬。结论：本研究结果可能表明，尼古丁以剂量依赖方式影响人间充质干细胞的生物物理特性，这可能使细胞对机械诱导和其他物理刺激的反应性降低。
