Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, AP, India.
Nanotechnology. 2012 Nov 16;23(45):455103. doi: 10.1088/0957-4484/23/45/455103. Epub 2012 Oct 12.
The biological approach to synthesis of AuNPs is eco-friendly and an ideal method to develop environmentally sustainable nanoparticles alternative to existing methods. We have developed a simple, fast, clean, efficient, low-cost and eco-friendly single-step green chemistry approach for the synthesis of biocompatible gold nanoparticles (AuNPs) from chloroauric acid (HAuCl(4)) using a water extract of Eclipta Alba leaves at room temperature. The AuNPs using Eclipta extract have been formed in very short time, even in less than 10 min. The as-synthesized AuNPs were thoroughly characterized by several physico-chemical techniques. The in vitro stability of as-synthesized AuNPs was studied in different buffer solutions. A plausible mechanism for the synthesis of AuNPs by Eclipta extract has been discussed. The biocompatibility of AuNPs was observed by in vitro cell culture assays. Finally, we have designed and developed a AuNPs-based drug delivery system (DDS) (Au-DOX) containing doxorubicin (DOX), a FDA approved anticancer drug. Administration of this DDS to breast cancer cells (MCF-7 and MDA-MB-231) shows significant inhibition of breast cancer cell proliferation compared to pristine doxorubicin. Therefore we strongly believe that the use of Eclipta Alba offers large-scale production of biocompatible AuNPs that can be used as a delivery vehicle for the treatment of cancer diseases.
从氯金酸 (HAuCl(4)) 出发，利用水飞蓟草叶的水提取物，在室温下，我们开发出了一种简单、快速、清洁、高效、低成本且环保的单步绿色化学方法，用于合成生物相容性的金纳米颗粒 (AuNPs)。利用水飞蓟草提取物，AuNPs 的形成时间非常短，甚至不到 10 分钟。使用多种物理化学技术对合成的 AuNPs 进行了彻底的表征。研究了在不同缓冲溶液中合成的 AuNPs 的体外稳定性。讨论了水飞蓟草提取物合成 AuNPs 的可能机制。通过体外细胞培养实验观察到 AuNPs 的生物相容性。最后，我们设计并开发了一种基于 AuNPs 的药物递送系统 (Au-DOX)，其中包含阿霉素 (DOX)，这是一种获得 FDA 批准的抗癌药物。与原始阿霉素相比，将这种 DDS 施用于乳腺癌细胞 (MCF-7 和 MDA-MB-231) 可显著抑制乳腺癌细胞的增殖。因此，我们坚信使用水飞蓟草可以大规模生产生物相容性的 AuNPs，可将其用作治疗癌症疾病的药物递送载体。
