Magadani Roshudufhadzwa, Ndinteh Derek Tantoh, Roux Saartjie, Nangah Louisiane Patrick, Atangwho Item Justin, Uti Daniel Ejim, Alum Esther Ugo, Egba Simeon Ikechukwu
Department of Chemical Science, Faculty of Science, University of Johannesburg, Doornfontein, 2028, South Africa.
Department of Physiology, Faculty of Science, Nelson Mandela University, Summerstrand, 6019, South Africa.
Anticancer Agents Med Chem. 2025;25(12):841-850. doi: 10.2174/0118715206325529241004064307.
The prevalent disease known as breast cancer has a significant impact on both men's and women's health and quality of life.
The aim of this study was to explore the potential roles of (planch.) extract and triterpenoid- derived gold nanoparticles (AuNPs) in cancer therapy, specifically targeting MCF-7 breast cancer cell lines.
Gold nanoparticles were synthesized utilizing triterpenoid (ZJ-AuNPs) and leaf extract from (LC-AuNPs). Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), High-resolution transmission electron microscopy (HRTEM), and UV-vis spectroscopy were employed to characterize the nanoparticles. Additionally, the MTT assay was used to assess the impact of AuNPs on cancer cell viability using MCF-7 breast cancer cell lines.
Analysis of ZJ-AuNPs and LC-AuNPs revealed DLS zeta potentials of -31.8 and -35.8 mV, respectively, and a corresponding UV-vis absorption maxima at 540 and 550 nm. Also, the ZJ-AuNPs and LC-AuNPs had respective zeta-sizes that ranged from 25.84 to 35.98 nm and polydispersive index values between 0.2360 and 0.773. Furthermore, the presence of the chemical groups -OH and -NH was shown to be necessary for the green method of capping and reducing the gold nanoparticles. Nevertheless, a significant decrease in cell viability percentages was noted in the MTT experiment, accompanied by an increase in the quantity or concentration of the nanoparticles for both ZJ-AuNPs and LC-AuNPs.
Given the data obtained in this study, the biosynthesized ZJ-AuNPs and LC-AuNPs were shown to possess potent cytotoxic effects on breast cancer cells. Hence, they may be valuable tools in the development of new cancer chemotherapy drugs.
乳腺癌这种常见疾病对男性和女性的健康及生活质量都有重大影响。
本研究的目的是探索（植物名称未给出）提取物和三萜类衍生金纳米颗粒（AuNPs）在癌症治疗中的潜在作用，特别是针对MCF - 7乳腺癌细胞系。
利用三萜类化合物（ZJ - AuNPs）和（植物名称未给出）叶提取物（LC - AuNPs）合成金纳米颗粒。采用傅里叶变换红外光谱（FTIR）、动态光散射（DLS）、高分辨率透射电子显微镜（HRTEM）和紫外可见光谱对纳米颗粒进行表征。此外，使用MTT法评估AuNPs对MCF - 7乳腺癌细胞系癌细胞活力的影响。
对ZJ - AuNPs和LC - AuNPs的分析显示，其DLS zeta电位分别为 - 31.8和 - 35.8 mV，相应的紫外可见吸收最大值分别在540和550 nm处。此外，ZJ - AuNPs和LC - AuNPs的zeta尺寸分别在25.84至35.98 nm范围内，多分散指数值在0.2360至0.773之间。此外，化学基团 - OH和 - NH的存在被证明是绿色包覆和还原金纳米颗粒方法所必需的。然而，在MTT实验中，细胞活力百分比显著下降，同时ZJ - AuNPs和LC - AuNPs的纳米颗粒数量或浓度增加。
根据本研究获得的数据，生物合成的ZJ - AuNPs和LC - AuNPs对乳腺癌细胞具有强大的细胞毒性作用。因此，它们可能是开发新型癌症化疗药物的有价值工具。
