Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, Ås, Norway.
Appl Environ Microbiol. 2013 Jan;79(1):48-56. doi: 10.1128/AEM.02464-12. Epub 2012 Oct 12.
Dietary inclusion of a bacterial meal has recently been shown to efficiently abolish soybean meal-induced enteritis in Atlantic salmon. The objective of this study was to investigate whether inclusion of this bacterial meal in the diet could abrogate disease development in a murine model of epithelial injury and colitis and thus possibly have therapeutic potential in human inflammatory bowel disease. C57BL/6N mice were fed ad libitum a control diet or an experimental diet containing 254 g/kg of body weight BioProtein, a bacterial meal consisting of Methylococcus capsulatus (Bath), together with the heterogenic bacteria Ralstonia sp., Brevibacillus agri, and Aneurinibacillus sp. At day 8, colitis was induced by 3.5% dextran sulfate sodium (DSS) ad libitum in the drinking water for 6 days. Symptoms of DSS treatment were less profound after prophylactic treatment with the diet containing the BioProtein. Colitis-associated parameters such as reduced body weight, colon shortening, and epithelial damage also showed significant improvement. Levels of acute-phase reactants, proteins whose plasma concentrations increase in response to inflammation, and neutrophil infiltration were reduced. On the other, increased epithelial cell proliferation and enhanced mucin 2 (Muc2) transcription indicated improved integrity of the colonic epithelial layer. BioProtein mainly consists of Methylococcus capsulatus (Bath) (88%). The results that we obtained when using a bacterial meal consisting of M. capsulatus (Bath) were similar to those obtained when using BioProtein in the DSS model. Our results show that a bacterial meal of the noncommensal bacterium M. capsulatus (Bath) has the potential to attenuate DSS-induced colitis in mice by enhancing colonic barrier function, as judged by increased epithelial proliferation and increased Muc2 transcription.
饮食中添加细菌餐最近已被证明能有效消除大豆蛋白引起的大西洋鲑鱼肠炎。本研究的目的是研究这种细菌餐是否能阻止上皮损伤和结肠炎的小鼠模型中的疾病发展，从而可能对人类炎症性肠病具有治疗潜力。C57BL/6N 小鼠自由喂食对照饮食或实验饮食，实验饮食中含有 254 g/kg 体重的 BioProtein，这是一种由 Methylococcus capsulatus（巴斯）组成的细菌餐，以及异养菌 Ralstonia sp.、Brevibacillus agri 和 Aneurinibacillus sp. 第 8 天，通过在饮用水中添加 3.5%葡聚糖硫酸钠（DSS）使小鼠自由摄入，持续 6 天诱导结肠炎。预防性喂食含有 BioProtein 的饮食后，DSS 处理的症状不太严重。与结肠炎相关的参数，如体重减轻、结肠缩短和上皮损伤也有明显改善。急性期反应物（炎症时血浆浓度增加的蛋白质）和中性粒细胞浸润水平降低。另一方面，上皮细胞增殖增加和粘蛋白 2（Muc2）转录增强表明结肠上皮层的完整性得到改善。BioProtein 主要由 Methylococcus capsulatus（巴斯）组成（88%）。当我们使用由 M. capsulatus（巴斯）组成的细菌餐时，我们得到的结果与在 DSS 模型中使用 BioProtein 时相似。我们的结果表明，非共生菌 M. capsulatus（巴斯）的细菌餐通过增强结肠屏障功能，具有减轻 DSS 诱导的小鼠结肠炎的潜力，这可以通过增加上皮细胞增殖和增加 Muc2 转录来判断。
