Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, China.
Arch Virol. 2013 Feb;158(2):387-97. doi: 10.1007/s00705-012-1495-4. Epub 2012 Oct 11.
Human enterovirus 71 (EV71) causes hand, foot and mouth disease in children under 6 years of age, and the neurological complications of this virus can lead to death. Until now, no vaccines or drugs have been available for the clinical control of this epidemic. Macrophages can engulf pathogens and mediate a series of host immune responses that play a role in the defence against infectious diseases. Using immunohistochemistry, we observed the localizations of virus in muscle tissues of EV71-infected mice. The macrophages isolated from the adult mice could kill the virus gradually in vitro, as shown using quantitative real-time PCR (qRT-PCR) and virus titration. Co-localisation of lysosomes and virus within macrophages suggested that the lysosomes were possibly responsible for the phagocytosis of EV71. Activation of the macrophages in the peritoneal cavity of mice four days pre-infection reduced the mortality of mice upon lethal EV71 infection. The adoptive transfer of macrophages from adult mice inhibited virus replication in the muscle tissues of infected mice, and this was followed by a relief of symptoms and a significant reduction of mortality, which suggested that the adoptive transfer of macrophages from adult humans represents a potential strategy to treat EV71-infected patients.
肠道病毒 71 型（EV71）可引起 6 岁以下儿童手足口病，该病毒的神经并发症可导致死亡。到目前为止，还没有针对这种疾病的临床控制疫苗或药物。巨噬细胞可以吞噬病原体，并介导一系列宿主免疫反应，在防御传染病中发挥作用。我们通过免疫组织化学观察到 EV71 感染小鼠肌肉组织中病毒的定位。从成年小鼠中分离出的巨噬细胞可以在体外逐渐杀死病毒，这可以通过实时定量 PCR（qRT-PCR）和病毒滴定法来证明。巨噬细胞内溶酶体和病毒的共定位表明溶酶体可能负责 EV71 的吞噬作用。在感染前四天对小鼠腹腔内巨噬细胞进行激活，可以降低致死性 EV71 感染后小鼠的死亡率。从成年小鼠中过继转移巨噬细胞可抑制感染小鼠肌肉组织中的病毒复制，随后症状缓解，死亡率显著降低，这表明从成年人类过继转移巨噬细胞可能是治疗 EV71 感染患者的一种潜在策略。
