用于快速基因组测序的文库制备和数据分析软件包。
Library preparation and data analysis packages for rapid genome sequencing.
作者信息
Pomraning Kyle R, Smith Kristina M, Bredeweg Erin L, Connolly Lanelle R, Phatale Pallavi A, Freitag Michael
机构信息
Department of Biochemistry and Biophysics, Center for Genome Research and Biocomputing (CGRB), Oregon State University, Corvallis, OR, USA.
出版信息
Methods Mol Biol. 2012;944:1-22. doi: 10.1007/978-1-62703-122-6_1.
High-throughput sequencing (HTS) has quickly become a valuable tool for comparative genetics and genomics and is now regularly carried out in laboratories that are not connected to large sequencing centers. Here we describe an updated version of our protocol for constructing single- and paired-end Illumina sequencing libraries, beginning with purified genomic DNA. The present protocol can also be used for "multiplexing," i.e. the analysis of several samples in a single flowcell lane by generating "barcoded" or "indexed" Illumina sequencing libraries in a way that is independent from Illumina-supported methods. To analyze sequencing results, we suggest several independent approaches but end users should be aware that this is a quickly evolving field and that currently many alignment (or "mapping") and counting algorithms are being developed and tested.
高通量测序（HTS）已迅速成为比较遗传学和基因组学的一种有价值的工具，目前在未与大型测序中心联网的实验室中也经常进行。在此，我们描述了一种从纯化的基因组DNA开始构建单端和双端Illumina测序文库的方案的更新版本。本方案也可用于“多重分析”，即通过以独立于Illumina支持方法的方式生成“条形码化”或“索引化”Illumina测序文库，在单个流动池泳道中分析多个样本。为了分析测序结果，我们建议了几种独立的方法，但终端用户应该意识到这是一个快速发展的领域，目前许多比对（或“映射”）和计数算法正在开发和测试。
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