Bills Gerald F, Dombrowski Anne W, Goetz Michael A
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Armilla, Granada, Spain.
Methods Mol Biol. 2012;944:79-96. doi: 10.1007/978-1-62703-122-6_5.
The FERMEX (Fermentation Extract) program was a highly successful source of microbial natural product molecules for pharmaceutical lead discovery. The program was based on the observation that solid fermentations of fungi generally exhibited more complex metabolite profiles than when the same strains were grown on liquid medium. To produce interference-free fermentations and improve organic product recovery, fungi colonized homogeneous media-saturated vermiculite thus promoting cellular and metabolic differentiation. Secondary metabolites in fungal cells were extracted from the substratum and medium with methyethylketone to generate metabolite-enriched screening samples. The necessary equipment, protocol, and media recipes are described along with examples of bioactive fungal metabolites produced in this system.
FERMEX（发酵提取物）项目是用于药物先导发现的微生物天然产物分子的一个非常成功的来源。该项目基于这样的观察结果：真菌的固体发酵通常比相同菌株在液体培养基上生长时表现出更复杂的代谢物谱。为了进行无干扰发酵并提高有机产物回收率，使真菌在均匀的、培养基饱和的蛭石上定殖，从而促进细胞和代谢分化。用甲乙酮从基质和培养基中提取真菌细胞中的次生代谢物，以生成富含代谢物的筛选样品。文中描述了必要的设备、方案和培养基配方，以及在该系统中产生的生物活性真菌代谢物的实例。
