Frisvad Jens C
Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Kongens Lyngby, Denmark.
Methods Mol Biol. 2012;944:47-58. doi: 10.1007/978-1-62703-122-6_3.
Growth media and incubation conditions have a very strong influence of secondary metabolite production. There is no consensus on which media are the optimal for metabolite production, but a series of useful and effective media and incubation conditions have been listed here. Chemically well-defined media are suited for biochemical studies, but in order to get chemical diversity expressed in filamentous fungi, sources rich in amino acids, vitamins, and trace metals have to be added, such as yeast extract and oatmeal. A battery of solid agar media is recommended for exploration of chemical diversity as agar plug samples are easily analyzed to get an optimal representation of the qualitative secondary metabolome. Standard incubation for a week at 25°C in darkness is recommended, but optimal conditions have to be modified depending on the ecology and physiology of different filamentous fungi.
生长培养基和培养条件对次级代谢产物的产生有非常强烈的影响。对于哪种培养基最适合代谢产物的产生，目前尚无共识，但这里列出了一系列有用且有效的培养基和培养条件。化学成分明确的培养基适用于生化研究，但为了使丝状真菌表达出化学多样性，必须添加富含氨基酸、维生素和微量金属的来源，如酵母提取物和燕麦片。推荐使用一系列固体琼脂培养基来探索化学多样性，因为琼脂塞样品易于分析，能够获得定性次级代谢组的最佳代表性结果。建议在黑暗中于25°C标准培养一周，但最佳条件必须根据不同丝状真菌的生态学和生理学进行调整。
