Instituto de Microelectrónica de Barcelona, IMB-CNM-CSIC, Universitat Autónoma de Barcelona, Barcelona, Spain.
Electrophoresis. 2012 Nov;33(21):3187-94. doi: 10.1002/elps.201200225. Epub 2012 Oct 12.
This work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells for multianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05-1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.
这项工作展示了一种基于全环烯烃聚合物的微流控器件的设计和制造，该器件能够在一系列金电极中捕获磁性珠并进行电化学检测。该芯片的尺寸为显微镜载玻片大小，具有六个独立的测量单元，用于多分析物检测目的。这项工作的目的是表明快速原型制作技术可以在新型生物测定法的开发中发挥作用，特别是在临床诊断应用中。我们展示了在临床相关的 0.05-1.0ng/mL 范围内成功测定肌钙蛋白-T（一种心脏疾病标志物）。该方法在 PBS 溶液中的检测限达到 0.017ng/mL，并且能够在 1:50 的人血清稀释度下检测到低于 1ng/mL 的浓度。
