Sadler D M, Reiss-Husson F, Rivas E
H.H. Wills Physics Laboratory, University of Bristol, ITL, U.K.
Chem Phys Lipids. 1990 Jan;52(1):41-8. doi: 10.1016/0009-3084(90)90005-c.
A method of deriving by neutron scattering thicknesses of lamellae in suspensions has been applied to single-walled vesicles of dimyristoyl phosphatidylcholine. The contrast variation method, based on data obtained for a range of isotope mixtures, has been used to extract a dimension Dw related to the lipid bilayer thickness and a measure alpha of the difference of density within the lamellae. Isotope mixtures for the lipid were used to optimize the information available. Dw is compared with results from multilayer stacks of lipid layers. The thickness for the low temperature L beta, structure has been observed to be higher than for the high temperature L alpha structure. Preliminary experiments on the kinetics of the mixing of the lipid isotope species are reported, and evidence is shown that the species are not segregated for lipids either above or below the transition temperature.
一种通过中子散射推导悬浮液中薄片厚度的方法已应用于二肉豆蔻酰磷脂酰胆碱的单壁囊泡。基于一系列同位素混合物获得的数据的对比变化法，已被用于提取与脂质双层厚度相关的尺寸Dw以及薄片内密度差异的量度α。脂质的同位素混合物用于优化可用信息。将Dw与脂质层多层堆叠的结果进行了比较。已观察到低温Lβ结构的厚度高于高温Lα结构的厚度。报道了关于脂质同位素物种混合动力学的初步实验，并表明在转变温度以上或以下的脂质中，这些物种没有分离。
