Eken Alper, Erdogan Seyda, Kuyucu Yurdun, Seydaoglu Gulsah, Polat Sait, Satar Nihat
Cukurova University, Faculty of Medicine, Department of Urology, Adana, Turkey;
Cukurova University, Faculty of Medicine, Department of Pathology, Adana, Turkey;
Can Urol Assoc J. 2013 May-Jun;7(5-6):E311-6. doi: 10.5489/cuaj.11293. Epub 2013 May 13.
We examine the ultrastructural configurations of Cajal cells by electron microscopy, as well as the quantitative changes occurring in Cajal cells by light microscopy.
In total, 35 patients with ureteropelvic junction (UPJ) obstruction and 7 patients without obstruction were compared immunohistochemically with c-kit (CD117) to quantify the number of cells. On electron microscopic examination, 7 patients with UPJ obstruction and 3 patients without obstruction were compared to evaluate the changes which occurred in the ultrastructural configuration of the Cajal cells.
On light microscopic examination, it was determined that the Cajal cells, which demonstrate c-kit (CD117) immunoreactive character, were located near the circular muscle layer and parallel to the muscle cells. The number of Cajal cells in the control group was significantly increased compared to the number of cells in patients with UPJ obstruction (p < 0.001). On electron microscopic examination, the number of interstitial cells was also higher in the control group. A decrease in the number of the caveolae in these cells was seen in the group with UPJ obstruction compared to the control group.
In UPJ obstruction, a decrease in the number of Cajal cells, as well as the changes in the morphologic structure of the Cajal cells, indicates that these cells have a role in the pacemaker system and are associated with ureteral peristalsis.
我们通过电子显微镜检查Cajal细胞的超微结构形态，以及通过光学显微镜观察Cajal细胞发生的数量变化。
总共35例肾盂输尿管连接部（UPJ）梗阻患者和7例无梗阻患者采用c-kit（CD117）免疫组织化学法进行比较，以量化细胞数量。在电子显微镜检查中，比较7例UPJ梗阻患者和3例无梗阻患者，以评估Cajal细胞超微结构形态发生的变化。
在光学显微镜检查中，确定显示c-kit（CD117）免疫反应性特征的Cajal细胞位于环形肌层附近且与肌细胞平行。与UPJ梗阻患者的细胞数量相比，对照组中Cajal细胞的数量显著增加（p < 0.001）。在电子显微镜检查中，对照组中间质细胞的数量也更高。与对照组相比，UPJ梗阻组中这些细胞的小凹数量减少。
在UPJ梗阻中，Cajal细胞数量的减少以及Cajal细胞形态结构的变化表明，这些细胞在起搏系统中起作用，并与输尿管蠕动相关。
