Wang Yubo, Wang Yong, Sun Jun, Dai Yue, Yang Fengyan, Jiang Hui, Irfan Muhammad, Chen Lijing
Key Laboratory of Agriculture Biotechnology, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
College of Chemical Engineering, University of Science and Technology Liaoning, Anshan, China.
Front Plant Sci. 2022 Jun 30;13:911203. doi: 10.3389/fpls.2022.911203. eCollection 2022.
Sieb.Zucc. is a fruit that is rich in flavonoids. Nevertheless, details of flavonoid formation and the potential mechanism behind flavonoid biosynthesis have not previously been reported. In order to explore the biosynthetic regulation mechanism of flavonoids in Sieb.Zucc., we conducted a combination of extensive targeted metabolite analysis and analyzed transcriptomes to determine the flavonoids present and the genes bound up with flavonoid biosynthesis in the two main cultivated varieties of Sieb.Zucc. in Northern China. The maturity period is from August to September. A total of 118 flavonoids were found in fruits. Among them, 39 flavonoids were accumulated at significant levels after fruit ripening. Transcriptome analysis indicated that most flavonoid biosynthesis structural genes and certain regulatory genes exhibited differential expression between the two varieties. Correlation analysis of transcriptome and metabolite profiles showed that the ways of expression of 21 differentially expressed genes related to structure and regulation between the 2 varieties were more highly correlated with 7 flavonoids after fruit ripening. These results contribute to the development of Sieb.Zucc. as a food and drug homologous functional food.
丝瓜是一种富含黄酮类化合物的果实。然而，黄酮类化合物的形成细节以及黄酮类生物合成背后的潜在机制此前尚未见报道。为了探究丝瓜中黄酮类化合物的生物合成调控机制，我们结合广泛的靶向代谢物分析并分析转录组，以确定中国北方两个主要栽培品种丝瓜中存在的黄酮类化合物以及与黄酮类生物合成相关的基因。成熟期为8月至9月。在果实中总共发现了118种黄酮类化合物。其中，39种黄酮类化合物在果实成熟后大量积累。转录组分析表明，大多数黄酮类生物合成结构基因和某些调控基因在两个品种之间表现出差异表达。转录组和代谢物谱的相关性分析表明，两个品种之间21个与结构和调控相关的差异表达基因的表达方式与果实成熟后7种黄酮类化合物的相关性更高。这些结果有助于将丝瓜开发为药食同源的功能性食品。
