Lachowicz Dominika, Skopińska Ewa, Pawłowska Jolanta, Kostkiewicz Marek, Obuch-Woszczatyński Piotr, Wultańska Dorota, Pituch Hanna
Katedra i Zakład Mikrobiologii Lekarskiej, Warszawskiego Uniwersytetu Medycznego.
Med Dosw Mikrobiol. 2012;64(2):115-22.
During the past 20 years, several studies at a national level in different countries followed resistance trends for Bacteroides sp. and Clostridium difficile. This study analysed antimicrobial susceptibility 73 anaerobic bacteria strains of Bacteroides fragilis group (BFG) and C. difficile to fluoroquinolones and other antimicrobial drugs.
The strictly anaerobes strains isolated in different hospitals were sent to the Department of Medical Microbiology, Medical Uniwersity of Warsaw, where species determination was carried out with the API20 ANA (bioMerieux SA, Marcy-l'Etoile, France) system. Susceptibility to antimicrobials was determined using E-test.
The rates of high resistance to ciprofloxacin and moxifloxacin of BFG was respectively 84% and 31% and among of C. difficile strains respectively 92% and 36%). The percentage of BFG strains resistant to erythromycin and clindamycin were respectively 84% and 46%. The percentage of C. difficile strains resistant to erythromycin and clindamycin was 52%. Reduced level of susceptibility of BFG strains to amoxicillin/clavulanic acid (8%) was confirmed. Resistance to cefoxitin was 16% of BFG strains. All tested strains as well as BFG and C. difficile were susceptible to metronidazole. Was observed reduced leve (EUCAST) of susceptibility of C. difficile strains to vancomycin (13%). CONCLUSIONS. Increasing resistance to various antimicrobial agents is a significant problem in Poland. This demonstrate the need to continue with antibiotic resistance testing and surveys in anaerobic bacteria.
在过去20年里，不同国家开展了多项国家级研究，追踪拟杆菌属和艰难梭菌的耐药趋势。本研究分析了73株脆弱拟杆菌群（BFG）和艰难梭菌厌氧菌株对氟喹诺酮类及其他抗菌药物的药敏情况。
将从不同医院分离出的严格厌氧菌菌株送至华沙医科大学医学微生物学系，使用API20 ANA（法国生物梅里埃公司，马西-埃图瓦勒）系统进行菌种鉴定。采用E-test法测定抗菌药物敏感性。
BFG对环丙沙星和莫西沙星的高耐药率分别为84%和31%，艰难梭菌菌株对二者的高耐药率分别为92%和36%。BFG菌株对红霉素和克林霉素的耐药率分别为84%和46%。艰难梭菌菌株对红霉素和克林霉素的耐药率为52%。证实BFG菌株对阿莫西林/克拉维酸的敏感性降低（8%）。BFG菌株对头孢西丁的耐药率为16%。所有受试菌株以及BFG和艰难梭菌对甲硝唑均敏感。观察到艰难梭菌菌株对万古霉素的敏感性降低（欧洲抗菌药物敏感性试验委员会标准）（13%）。结论：在波兰，对各种抗菌药物的耐药性增加是一个重大问题。这表明有必要继续对厌氧菌进行抗生素耐药性检测和调查。
