Department of Forest and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-kibanadai-nishi, Miyazaki 889-2192, Japan.
Bioresour Technol. 2012 Dec;126:137-41. doi: 10.1016/j.biortech.2012.09.007. Epub 2012 Sep 13.
We propose a new process of unified aerobic delignification and anaerobic saccharification and fermentation of wood by a single microorganism, the white-rot fungus Phlebia sp. MG-60. This fungus is able to selectively degrade lignin under aerobic solid state fermentation conditions, and to produce ethanol directly from delignified oak wood under semi-aerobic liquid culture conditions. After 56 d aerobic incubation, 40.7% of initial lignin and negligible glucan were degraded. Then under semi-aerobic conditions without the addition of cellulase, 43.9% of theoretical maximum ethanol was produced after 20 d. Changing from aerobic conditions (biological delignification pretreatment) to semi-aerobic conditions (saccharification and fermentation) enabled the fermentation of wood by solely biological processes. This is the first report of ethanol production from woody biomass using a single microorganism without addition of chemicals or enzymes.
我们提出了一种由单一微生物——白腐菌 Phlebia sp. MG-60 实现木材有氧脱木质素和无氧糖化发酵统一的新工艺。该真菌能够在有氧固态发酵条件下选择性地降解木质素，并在半需氧液体培养条件下直接从脱木质素的橡木中生产乙醇。经过 56 天的有氧孵育，初始木质素的 40.7%和几乎可以忽略不计的纤维素被降解。然后在无需添加纤维素酶的半需氧条件下，经过 20 天可生产出 43.9%的理论最大乙醇产量。从有氧条件（生物脱木质素预处理）转变为半需氧条件（糖化和发酵）使得仅通过生物过程即可发酵木材。这是首次报道使用单一微生物在不添加化学物质或酶的情况下从木质生物质生产乙醇。
