Sharma Veena, Paliwal Ritu, Janmeda Pracheta, Sharma Shatruhan
Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India.
Zhong Xi Yi Jie He Xue Bao. 2012 Oct;10(10):1171-8. doi: 10.3736/jcim20121015.
To investigate the potential of hydroethanolic extract of Moringa oleifera (MOHE) against 7, 12-dimethylbenz [a] anthracene (DMBA)-induced toxicity in male Swiss albino mice.
Experimental mice were respectively pretreated with 200 and 400 mg/kg of MOHE, and 0.5% and 1% of butylated hydroxyanisole (BHA) for two weeks prior to the administration of 15 mg/kg of DMBA, respectively. Levels of xenobiotic metabolizing enzymes such as cytochrome (Cyt) P450 and Cyt b5, activities of reduced glutathione (GSH) and glutathione-S-transferase (GST) and renal aspartate amino transaminase (AST), alanine amino transaminase (ALT) and alkaline phosphatase (ALP), and content of protein and total cholesterol were measured to determine the nephrotoxicity caused by DMBA and to elucidate the ameliorating role of M. oleifera.
Single oral administration of 15 mg/kg of DMBA resulted in significant increases in Cyt P450 and Cyt b5 (P<0.01). The toxic effect of DMBA was justified by the significant decreases in the activities of GSH and GST in renal tissues (P<0.05). The levels of renal AST, ALT and ALP and protein content which are indicative of renocellular damage were also found decreased along with significant increase in total cholesterol content in DMBA-treated mice (P<0.01). The DMBA-induced alterations in the tissues were significantly reversed after pretreatment with 200 and 400 mg/kg of MOHE orally for 14 d (P<0.01).
The effects of MOHE in enhancing the levels of antioxidants and enhancing the levels of biochemical assays in DMBA-induced carcinogenesis are by reducing the formation of free radicals. This study rationalizes the ethnomedicinal use of M. oleifera for the protection against nephrotoxicity induced by chemical carcinogens.
研究辣木乙醇提取物（MOHE）对7,12-二甲基苯并[a]蒽（DMBA）诱导的雄性瑞士白化小鼠毒性的潜在作用。
实验小鼠分别在给予15mg/kg DMBA前两周，分别用200和400mg/kg的MOHE以及0.5%和1%的丁基羟基茴香醚（BHA）进行预处理。测量异源物质代谢酶如细胞色素（Cyt）P450和Cyt b5的水平、还原型谷胱甘肽（GSH）和谷胱甘肽-S-转移酶（GST）的活性以及肾天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP），并测定蛋白质和总胆固醇含量，以确定DMBA引起的肾毒性，并阐明辣木的改善作用。
单次口服15mg/kg DMBA导致Cyt P450和Cyt b5显著增加（P<0.01）。肾组织中GSH和GST活性的显著降低证明了DMBA的毒性作用（P<0.05）。在DMBA处理的小鼠中，表明肾细胞损伤的肾AST、ALT和ALP水平以及蛋白质含量也发现降低，同时总胆固醇含量显著增加（P<0.01）。在用200和400mg/kg的MOHE口服预处理14天后，DMBA诱导的组织改变得到显著逆转（P<0.01）。
MOHE在DMBA诱导的致癌过程中增强抗氧化剂水平和生化检测水平的作用是通过减少自由基的形成。本研究使辣木在预防化学致癌物诱导的肾毒性方面的民族药用合理化。
